Activity and Properties of 5- Nucleotidase in Human Amniotic Fluid | ||
| Rafidain Journal of Science | ||
| Article 3, Volume 20, Issue 5, July 2009, Pages 25-34 PDF (0 K) | ||
| DOI: 10.33899/rjs.2009.40029 | ||
| Authors | ||
| Raja A. AL-Taii; Muna H. Jankeer | ||
| Abstract | ||
| The present research includes a study of activity and properties of 5 - Nucleotidase (5-NT, EC.3.1.3.5) was determined in human amniotic fluid using coloremetric method. Maximal activity of the enzyme was obtained in a reaction mixture containing 40 mM of Barbiturate-HCl buffer at pH 7.5 containing 11 mM of adenosine 5- monophosphate (5-AMP) as a substrate and 50 l of amniotic fluid (Enzyme volume). The reaction mixture was incubated at 37C for 30min, under the optimum condition. The enzyme activity equal to (66.7±0.32) unit per liter in amniotic fluid. | ||
| Keywords | ||
| Nucleotidase; Adenosine; monophosphate; Human amniotic fluid | ||
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