Effect of Aqueous Extracts of Nerium oleander and Melia azedarach on Growth Leishmania tropica promastigotes In Vitro | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 14, Issue 2, August 2009, Pages 42-45 | ||
| Author | ||
| Shayma Abdullah Al-Jubouri | ||
| Abstract | ||
| The present study tacked the effect of the aqueous plant extracts of Melia azedarach fruits and Nerium oleander leaves and fruits on Leishmania tropica promastigotes growing on Tobie's medium to which 15 % of human blood is added. The results indicated that these extracts have poisonous effect on the promastigotes of L. tropica. The used concentrations were 0.75, 1, 1.5, 2.5, 5 mg/ml for fruits extract of M. azedarach, 0.05, 0.25, 0.5, 1, 2.5 mg/ml for leaves extract of N. oleander and 0.5, 1, 1.75, 2.5, 5 mg/ml for fruits extract of N. oleander, and caused gradual inhibition of promastigotes number with the increase of concentration through different growth periods 24, 48, 72, 96 hours. The high concentrations 5 mg/ml for fruits of M. azedarach 2.5 mg/ml for leaves of N. oleander and 5 mg/ml for fruits of N. oleander caused inhibition of the promastigotes growth of L. tropica at rates 97 % , 71.9 % and 81.5 respectively during 96 hours of growth. The IC50 concentration for extracts were determined which were 0.75, 1, 1.75 mg/ml for M. azedarach fruits, N. oleander leaves and fruits extracts respectively during 96 hour of growth. | ||
| Keywords | ||
| Leishmania; Plant Extracts | ||
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