Comparison of Classical and Molecular Identification for Tannerella forsythia from Chronic Periodontitis Infections | ||
| Rafidain Journal of Science | ||
| Article 7, Volume 23, Issue 2, March 2012, Pages 79-90 PDF (0 K) | ||
| DOI: 10.33899/rjs.2012.27997 | ||
| Author | ||
| Sahira I. AL-Sanjary | ||
| Abstract | ||
| The study included (48) Chronic Periodontitis samples were collected from both sexes with ages between (20-60) years, in addition to determination the average of pocket depth. Morphological and Classical characteristics of Tannerella forsythia using light and fluorescent microscopy were carried , in addition to biochemical tests. The species shown to be catalase and indol negative and without acid from glucose but Esculin hydrolysis test was positive while no growth was noted on esculin bile salt medium. DNA was extracted from Tannerella forsythia using the boiling method approximately (5.2-6.5) µg/ µl was obtained with (1.6-1.8) purity. polymerase chain reaction using species specific primer to amplify 641bp fragment from 16SrRNA gene was performed. It was found to be a more effective method for the detection of Tannerella forsythia in clinical specimen as it gave (43.7) positive isolates compared with (20.8) by using conventional methods. | ||
| Keywords | ||
| periodontitis; Tannerella forsythia; PCR | ||
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