Evaluation of Crude Anthrax Protective Antigen as an Adjutant For Brucella Abortus Vaccine In Mice | ||
| Engineering and Technology Journal | ||
| Article 1, Volume 30, Issue 3, January 2012, Pages 500-511 PDF (312.73 K) | ||
| DOI: 10.30684/etj.30.3.13 | ||
| Author | ||
| Inam Jasim Lafta | ||
| Abstract | ||
| Anthrax crude protective antigen (PA) was used in this study as an adjutant with Brucella abortus S19 strain vaccine in a ratio of 1:1 to immunize mice in dose of 0.1 ml s/c in order to decrease side effects of S19 vaccine and also to enhance its immunogenicity. Three groups of mice were immunized with the mixture (composed of anthrax PA +S19, S19 alone, and sterile PBS solution as a control. They were compared concerning cellular and humeral immunity. The group treated with the mixture was the best regarding skin test, the difference of thickness was 1.51± 0.103 mm after 24 h and 1.14± 0.103 mm and 0.89± 0.100 mm after 48 and 72 h, respectively. As well as that group revealed the highest antibody titer, which reported 0.7153± 0.08 nm as compared with 0.3743± 0.06 nm in S19 group. These findings were verified by studying the histopathological changes. | ||
| Keywords | ||
| Brucella abortus; Vaccine; Anthrax; Protective antigen; adjuvant | ||
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