EVALUATING THE VIRULENCE OF SOME PATHOGENIC ISOLATES FOR THREE FUSARIUM SPECIES IN DATE-PALM AND THEIR CONTROL. | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 43, Issue 2, June 2013, Pages 7-17 | ||
| Author | ||
| Kamil S. Juber | ||
| Abstract | ||
| This research aims to evaluate the virulence of some Fusarium species isolates which were associated with declined date-palms in Babylon governorate and control the pathogen by using the biocontrol agent Trichoderma harzianum and the fungicide Beltanol. The preliminary tests of pathogenicity for isolates of Fusarium spp. showed that all the isolates were significantly reduce cabbage seed germination percentage. The isolates Fo9, Fss3 and Fg4 achieved the highest reduction in seed germination, the percentage of seed germination in their treatment was 0.00-4.00% and all the other isolates were pathogenic and reduced the seed germination to 6.66-40.00% compared with control 82.66%. Results of influence of the pathogenic isolates in 3 months date-palm seedlings under lathhouse conditions indicated that all tests isolates were pathogenic compared with the control (without pathogen) and caused 73.33-93.33% infection severity. Under the lathhouse conditions, the results of test for effect of chemical fungicide (Beltanol) and biocontrol agent T. harzianum on shoot & root length and fresh & dry weight of date-palm seedlings 6 months old showed that all treatment caused significant reduction in percentage of infection severity on 6 month date-palm seedlings compared with pathogenic fungi treatments Fo9, Fs3 and Fg4 which showed 73.33%, 80.00% and 86.66% infection severity respectively. Beltanol with pathogenic fungi treatment Fo9, Fs3 and Fg4 was superior to other treatments 20.00%, 13.33% and 6.66% followed by the T. harzianum with pathogenic fungi Fo9, Fs3 and Fg4 40.00%, 26.66% and 26.66%. Also all treatments indicated that significant increase in all test palnts growth average compared with control treatment. But the treatment of the T. harzianum alone is in the first in significant increase in all test plants growth average. Results of the field experiment were similar to the lathhouse experiment, all the treatments that used to control pathogen indicated significant reduction in infection by date-palm declined disease pathogen on 3 years offshoot, it was 13.33-40.00% compared with control treatment with pathogenic fungi only which was 73.33%-80.00%. | ||
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