H. Hanoush, N. (2023). ANTIFUNGAL POTENTIAL OF ALCOHOLIC PLANT EXTRACTS IN REDUCING FUNGAL CONTAMINATION IN PHOENIX DACTYLIFERA TISSUE CULTURE. Alustath, 21(2), 467-479. doi: 10.32649/ajas.2024.144138.1092
N. H. Hanoush. "ANTIFUNGAL POTENTIAL OF ALCOHOLIC PLANT EXTRACTS IN REDUCING FUNGAL CONTAMINATION IN PHOENIX DACTYLIFERA TISSUE CULTURE". Alustath, 21, 2, 2023, 467-479. doi: 10.32649/ajas.2024.144138.1092
H. Hanoush, N. (2023). 'ANTIFUNGAL POTENTIAL OF ALCOHOLIC PLANT EXTRACTS IN REDUCING FUNGAL CONTAMINATION IN PHOENIX DACTYLIFERA TISSUE CULTURE', Alustath, 21(2), pp. 467-479. doi: 10.32649/ajas.2024.144138.1092
H. Hanoush, N. ANTIFUNGAL POTENTIAL OF ALCOHOLIC PLANT EXTRACTS IN REDUCING FUNGAL CONTAMINATION IN PHOENIX DACTYLIFERA TISSUE CULTURE. Alustath, 2023; 21(2): 467-479. doi: 10.32649/ajas.2024.144138.1092

ANTIFUNGAL POTENTIAL OF ALCOHOLIC PLANT EXTRACTS IN REDUCING FUNGAL CONTAMINATION IN PHOENIX DACTYLIFERA TISSUE CULTURE

ANBAR JOURNAL OF AGRICULTURAL SCIENCES
Article 20, Volume 21, Issue 2, November and December 2023, Pages 467-479    PDF (613.11 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2024.144138.1092
Author
N. H. Hanoush*
Scientific Affairs, University Headquarter, University of Anbar, Ramadi, Iraq
Abstract
This study was conducted to investigation was carried out to evaluate the efficacy of certain plant extracts derived from pomegranate peels and black seeds in combating fungal issues in tissue culture, exploring their potential as natural substitutes for chemical treatments.
The study was conducted in the fungi lab, University of Anbar, 2022-2023. In this study, three types of fungi were isolated from the plant tissues. Alcoholic extracts of pomegranate peels and black seed were used at different concentrations 0, 5, 10, 15 and 20% to inhibiting the growth of fungi isolated from tissue culture in PDA medium.
The study results showed the isolation of three types of fungi associated with culture of plant tissues in vitro (Alternaria alternata, Asperigellus niger and Fusarium solani). The results showed the highest rate of growth inhibition at a 20% concentration, the alcoholic extracts of pomegranate peel and black seeds of isolates of fungi A. alternata, A. niger and F. solani were (73.89 and 73.63)%, (74.99 and 74.78)%, (77.42 and 78.00)%, respectively. The extracts from black seed and pomegranate peels exhibit the most effective fungistatic properties. These plants possess medicinal qualities, including antioxidant, antimicrobial, and anti-inflammatory attributes, largely attributed to their polyphenolic compounds. So that, can used these extracts to control on pathogen fungi to plant.
Keywords
Alcoholic extracts; Fungi; Tissue culture; Pomegranate peel
References
  1. Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. J. econ. Entomol, 18(2): 265-267.
  2. Al-Hasany, A. R., Alhilfi, S. K., and Alfarjawi, T. M. (2020). Effect of foliar feeding with nano-boron on the growth and yield of two cultivars of faba bean crop (Vicia faba L.). International Journal of Agricultural and Statistical Sciences, 16(1): 237-241.
  3. Al-Nadabi, H. H., Al-Buraiki, N. S., Al-Nabhani, A. A., Maharachchikumbura, S. N., Velazhahan, R., and Al-Sadi, A. M. (2021). In vitro antifungal activity of endophytic bacteria isolated from date palm (Phoenix doctylifera L.) against fungal pathogens causing leaf spot of date palm. Egyptian Journal of Biological Pest Control, 31: 1-8.‏
  4. Ati, M. A. Z., and Ahmed, A. N. (2022). The effect of some alcoholic extracts of some plants in reducing the infection of fungi associated with plant tissue culture. International Journal of Agricultural and Statistical Sciences, 18(1): 57-63.‏
  5. Barnett, H. L., and Hunter, B. B. (1972). Illustrated genera of imperfect fungi. (3rd ed). Burgess Publishing Company, Minnesota. U.S.A.
  6. Bhatt, L. R., Lim, J. A., and Baek, S. H. (2005). Antimicrobial activity of Nepalese medicinal plants on skin microbial inflammation pathogens. Journal of Cosmetic and Public Health, 1: 6-9.
  7. Dammak, I., Hamdi, Z., El Euch, S. K., Zemni, H., Mliki, A., Hassouna, M., and Lasram, S. (2019). Evaluation of antifungal and anti-ochratoxigenic activities of Salvia officinalis, Lavandula dentata and Laurus nobilis essential oils and a major monoterpene constituent 1, 8-cineole against Aspergillus carbonarius. Industrial Crops and Products, 128: 85-93.‏
  8. Daniel, C. K., Lennox, C. L., and Vries, F. A. (2015). In-vitro effects of garlic extracts on pathogenic fungi Botrytis cinerea, Penicillium expansum and Neofabraea alba. South African Journal of Science, 111(7-8): 1-8.
  9. Domsch, K. H., Gams, W., and Anderson, T. H. (1980). Compendium of soil fungi. Volume 1. Academic Press (London) Ltd.
  10. Dushyent, G., and Bohra, A. (1997). Effect of extracts of some halophytes on the growth of Alternaria solani. Journal of Mycological Plant Pathology, 27: 233.
  11. Ellis, M. B. (1976). More dematiaceous hyphomycetes. Common. Mycol. Inst. England.
  12. El-Kalamouni, C., Venskutonis, P. R., Zebib, B., Merah, O., Raynaud, C., and Talou, T. (2017). Antioxidant and antimicrobial activities of the essential oil of Achillea millefolium L. grown in France. Medicines, 4(2): 30.
  13. Guo, G., Wang, H. X., and Ng, T. B. (2009). Pomegranin, an antifungal peptide from pomegranate peels. Protein and Peptide Letters, 16(1): 82-85.
  14. Hamim, S. S. (2003). Efficacy of some plant extracts against common pathogens in bacterial skin infections. Master’s Thesis, College of Education, University of Basra.
  15. Harbourne, J. B. C. (1973). Phytochemical, methods Chpman and Hall. London, 226.
  16. Harbone, J. B. (1994). Phytochemical methods 2nd edition London.
  17. Hussain, K. A. (2023). Effects of some plants extracts in growth of some dermatophytes. Journal of University of Kerbala, 20(1): 99-114.
  18. Jassim, N. S. (2017). Evalution of some plant extract against the growth of Alternaria alternata as one of the causal agent of leaf spots disease of Date Palm Phoenix dactylifera L. Basrah Journal for Date Palm Research, 16(1): 75-91. ‏
  19. Jiménez-Reyes, M. F., Carrasco, H., Olea, A. F., and Silva-Moreno, E. (2019). Natural compounds: A sustainable alternative to the phytopathogens control. Journal of the Chilean Chemical Society, 64(2): 4459-4465.
  20. Kursa, W., Jamiołkowska, A., Wyrostek, J., and Kowalski, R. (2022). Antifungal effect of plant extracts on the growth of the cereal pathogen fusarium spp.—an in vitro study. Agronomy, 12(12): 3204.‏
  21. Matrood, A. A. A., Valdespino, C. A. R., Al-Waeli, M. A., Khrieba, M. I., and Rhouma, A. (2021). Pathogenicity and chemical control of Alternaria sp. on date palm (Phoenix dactylifera L.). Plant Science Today, 8(2): 386-391.‏
  22. Molnar, M., Jerković, I., Suknović, D., Bilić Rajs, B., Aladić, K., Šubarić, D., and Jokić, S. (2017). Screening of six medicinal plant extracts obtained by two conventional methods and supercritical CO2 extraction targeted on coumarin content, 2, 2-diphenyl-1-picrylhydrazyl radical scavenging capacity and total phenols content. Molecules, 22(3): 348.‏
  23. Mutlu-Ingok, A., Devecioglu, D., Dikmetas, D. N., Karbancioglu-Guler, F., and Capanoglu, E. (2020). Antibacterial, antifungal, antimycotoxigenic, and antioxidant activities of essential oils: An updated review. Molecules, 25(20): 4711.
  24. Noaema, A. H., AlKafaji, M. H., and Alhasany, A. R. (2020). Effect of nano-fertilization on growth and yield of three varieties of wheat bread (Triticum Aestivum L.). International Journal of Agricultural and Statistical Sciences, 16(1): 1269-1274.
  25. Obeid, Z. H., N. Mahmoud, and A. Mezher. (2013). Effect of aqueous extract of pomegranate and mint on the activity and growth of the fungus Alternaria alternate. Journal of University of Babylon for Pure and Applied Sciences, 21(3): 892-901.
  26. Poulios, E., Giaginis, C., and Vasios, G. K. (2019). Current advances on the extraction and identification of bioactive components of sage (Salvia spp.). Current Pharmaceutical Biotechnology, 20(10): 845-857.‏
  27. Rowshan, V., and Najafian, S. (2020). Polyphenolic contents and antioxidant activities of aerial parts of Salvia multicaulis from the Iran flora. Natural product research, 34(16): 2351-2353.
  28. Safari, M. R., Azizi, O., Heidary, S. S., Kheiripour, N., and Ravan, A. P. (2018). Antiglycation and antioxidant activity of four Iranian medical plant extracts. Journal of pharmacopuncture, 21(2): 82.‏
  29. Shoaib, A., Akhtar, M., Javaid, A., Ali, H., Nisar, Z., and Javed, S. (2021). Antifungal potential of zinc against leaf spot disease in chili pepper caused by Alternaria alternata. Physiology and Molecular Biology of Plants, 27(6): 1361-1376.‏
  30. Wens, A., and Geuens, J. (2022). In vitro and in vivo antifungal activity of plant extracts against common phytopathogenic fungi. Journal of BioScience and Biotechnology, 11(1): 15-21.
  31. Yamin, S., and Kurniawan, H. (2009). SPSS complete: Teknik analisis statistik terlengkap dengan software SPSS. Jakarta: Salemba Infotek.‏
  32. Yilar, M., Kadioglu, I., and Telci, I. (2018). Chemical Composition and Antifungal activity of Salvia Officinalis (L.), S. Cryptantha (Montbret Et Aucher Ex Benth.), S. Tomentosa (Mıll.) Plant ossential oils and extracts. Fresenius Environmental Bulletin,‏ 27(3): 1695-1706.
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