Gh. Sarhan, I., I. Al-Hadidy, Y., F. Weshah, S. (2023). EFFECT OF GUNDELIA TOURNEFORTII L. EXTRACT ON LIPID PROFILE AND FLORA BALANCE IN RATS EXPOSED TO EXPERIMENTAL PSEUDOMONAS AERUGINOSA INFECTION. Alustath, 21(2), 366-376. doi: 10.32649/ajas.2023.181867
I. Gh. Sarhan; Y. I. Al-Hadidy; Sh. F. Weshah. "EFFECT OF GUNDELIA TOURNEFORTII L. EXTRACT ON LIPID PROFILE AND FLORA BALANCE IN RATS EXPOSED TO EXPERIMENTAL PSEUDOMONAS AERUGINOSA INFECTION". Alustath, 21, 2, 2023, 366-376. doi: 10.32649/ajas.2023.181867
Gh. Sarhan, I., I. Al-Hadidy, Y., F. Weshah, S. (2023). 'EFFECT OF GUNDELIA TOURNEFORTII L. EXTRACT ON LIPID PROFILE AND FLORA BALANCE IN RATS EXPOSED TO EXPERIMENTAL PSEUDOMONAS AERUGINOSA INFECTION', Alustath, 21(2), pp. 366-376. doi: 10.32649/ajas.2023.181867
Gh. Sarhan, I., I. Al-Hadidy, Y., F. Weshah, S. EFFECT OF GUNDELIA TOURNEFORTII L. EXTRACT ON LIPID PROFILE AND FLORA BALANCE IN RATS EXPOSED TO EXPERIMENTAL PSEUDOMONAS AERUGINOSA INFECTION. Alustath, 2023; 21(2): 366-376. doi: 10.32649/ajas.2023.181867

EFFECT OF GUNDELIA TOURNEFORTII L. EXTRACT ON LIPID PROFILE AND FLORA BALANCE IN RATS EXPOSED TO EXPERIMENTAL PSEUDOMONAS AERUGINOSA INFECTION

ANBAR JOURNAL OF AGRICULTURAL SCIENCES
Article 10, Volume 21, Issue 2, November and December 2023, Pages 366-376    PDF (379.78 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2023.181867
Authors
I. Gh. Sarhan1; Y. I. Al-Hadidy1; Sh. F. Weshah2
1Tikrit University - College of Agricultural
2College of Health and Medica Techniques - Al-Dour, Northern Technical University, Tikrit, Iraq
Abstract
The study aimed to investigate the effect of alcoholic Gundelia tourneofortti L. extract on lipid profile and the balance of microbial flora in the intestines of laboratory rats when infected with Pseudomonas aeroginosa. The study was used 20 laboratory rats (Albino Sprague - Dawley) at the age of 7-8 weeks, with an average weights 143-148 gm, divided into four groups, with five replications, and the inhibition activity Pseudomonas aeruginosa was studied by the concentrations of 200, 400 and 600 µg/ml of the extract using the well diffusion assay method. The results found an increase in the alcoholic extraction effectiveness by increasing its concentration and that the alcoholic extraction was the most effective compared with aqueous extract, with the highest inhibition diameter of 30 mm at a concentration of 600 µg/ml. The results also show an increase (P<0.05) in the level of total cholesterol, triglycerides and LDL of groups of rats infected with Pseudo. aeruginosa was 70.5, 73.0, and 14.2 mg/dl, respectively and HDL levels decreased at 27.0 mg/dl, compared with the control group. As for the effect of infection with Pseudo. aeruginosa on the balance of normal flora in rats, it caused a significant decrease at (P<0.05) in the total number of intestinal bacteria, while the total number of LAB bacteria decreased compared to the control group rats.
The oral administration of the alcoholic extract at a concentration of 230 and 430 µg/kg with infection has a significant effect in reducing the negative effect of groups infected with Pseudo. aeruginosa on all the above-measured parameter.
Keywords
Alcoholic extract; Gundelia tourneofortti L; Pseudomonas aeroginosa; Lipid profile; Micro flora
References
  1. Akyildiz S., Denli M., Alp S. Y., and Cardozo P. W. (2016). Effects of Dietary Addition of Plant Extract on Growth Performance, Intestinal Microflora and Serum Biochemistry in Broilers. 1st International Animal Nutrition Congress, September 28th October, Antalya/Turkey), 249-252.
  2. Akyildiz, S., Ozcan, N., and Denli, M. (2017). Efficacy of herbal extracts on growth performance, serum biochemistry and intestinal selected bacterial population in broilers. Scientific Papers: Series D, Animal Science-The International Session of Scientific Communications of the Faculty of Animal Science, 61-65.
  3. Al-Fahdawi, A. A. S. (2017). Effect of physical, chemical and tissue for the production of Gundelia tournefortii heaves and the treatment of the rats affected by Pseudomonas aeruginosa. Master's Thesis, Tikrit University.
  4. Al-Janabi, J. K., and S. Abdel-Amir. (2014). Evaluation of the efficiency of green tea extracts and scholars in the growth of fungi, College of Science, University of Babylon.
  5. Al-Younis, N. K., and Argushy, Z. M. (2009). Antibacterial evaluation of some medicinal plants from Kurdistan region. Journal Duhok Univ, 12(1): 256-261.
  6. Apak, R., Güçlü, K., Demirata, B., Özyürek, M., Çelik, S. E., Bektaşoğlu, B., ... and Özyurt, D. (2007). Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules, 12(7): 1496-1547.
  7. Asadi-Samani, M., Rafieian-Kopaei, M., and Azimi, N. (2013). Gundelia: a systematic review of medicinal and molecular perspective. Pakistan journal of biological sciences: Pakistan Journal of Biological Sciences, 16(21): 1238-1247.
  8. Azadmehr, A., Hajiaghaee, R., Afshari, A., Amirghofran, Z., Refieian-Kopaei, M., Yousofi Darani, H., and Shirzad, H. (2011). Evaluation of in vivo immune response activity and in vitro anti-cancer effect by Scrophularia megalantha. Journal of Medicinal Plants Research, 5(11): 2365-2368.
  9. Azeez, O. H., and Kheder, A. E. (2012). Effect of Gundelia tournefortii on some biochemical parameters in dexamethasone-induced hyperglycemic and hyperlipidemic mice. Iraqi Journal of Veterinary Sciences, 26(2): 73-79.
  10. Balouiri, M., Sadiki, M., and Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis, 6(2): 71-79.
  11. Boi, M. (2012). The Ethnocultural significance for the use of plants in Ancient Funerary Rituals and its possible implications with pollens found on the Shroud of Turin. In Ben Congreso Internacional sobre la Sábana Santa en España, 15.
  12. Cakilcioglu, U., and Khatun, S. (2011). Nitrate, moisture and ash contents of edible wild plants. J Cell Plant Sci, 2(1): 1-5.
  13. Ceylan, S., Cetin, S., Camadan, Y., Saral, O., Ozsen, O., and Tutus, A. (2019). Antibacterial and antioxidant activities of traditional medicinal plants from the Erzurum region of Turkey. Irish Journal of Medical Science (1971-): 188, 1303-1309.
  14. Cha, D., and Park, Y. (2019). Association between dietary cholesterol and their food sources and risk for hypercholesterolemia: the 2012–2016 Korea national health and nutrition examination survey. Nutrients, 11(4): 846.
  15. Dogan, Y., Baslar, S., Ay, G., and Mert, H. H. (2004). The use of wild edible plants in western and central Anatolia (Turkey). Economic botany, 58(4): 684-690.
  16. Duncan, D. B. (1955). Multiple range and multiple. F-test Biometrics, 11(1): 1-42.
  17. Gholami-Ahangaran, M., Ahmadi-Dastgerdi, A., and Karimi-Dehkordi, M. (2020). Thymol and carvacrol; as antibiotic alternative in green healthy poultry production. Plant Biotechnol Persa, 2(1): 22-25.
  18. Gholami‐Ahangaran, M., Karimi‐Dehkordi, M., Akbari Javar, A., Haj Salehi, M., and Ostadpoor, M. (2021). A systematic review on the effect of Ginger (Zingiber officinale) on improvement of biological and fertility indices of sperm in laboratory animals, poultry and humans. Veterinary medicine and science, 7(5): 1959-1969.
  19. Greenwell, M., and Rahman, P. K. S. M. (2015). Medicinal plants: their use in anticancer treatment. International journal of pharmaceutical sciences and research, 6(10): 4103-4112.
  20. Halabi, S., Battah, A. A., Aburjai, T., and Hudaib, M. (2005). Phytochemical and Antiplatelet Investigation of Gundelia tournifortii. Pharmaceutical biology, 43(6): 496-500.
  21. Hernández-Pérez, M., López-García, R. E., Rabanal, R. M., Darias, V., and Arias, A. (1994). Antimicrobial activity of Visnea mocanera leaf extracts. Journal of ethnopharmacology, 41(1-2): 115-119.
  22. Islam, M. T., Ali, E. S., and Mubarak, M. S. (2020). Anti‐obesity effect of plant diterpenes and their derivatives: a review. Phytotherapy research, 34(6): 1216-1225.
  23. Jayaraj, R., Deb, U., Bhaskar, A. S. B., Prasad, G. B. K. S., and Rao, P. L. (2007). Hepatoprotective efficacy of certain flavonoids against microcystin induced toxicity in mice. Environmental Toxicology: An International Journal, 22(5): 472-479.
  24. Kadan, S., Rayan, M., and Rayan, A. (2013). Anticancer activity of anise (Pimpinella anisum L.) seed extract. The Open Nutraceuticals Journal, 6(1).
  25. Karaaslan, Ö., Çöteli, E., and Karataş, F. (2014). Investigation of amounts of A, E, C vitamins with malondialdehyde and glutathione in plant Gundelia tournefortii. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 7(2): 159-168.
  26. Karimi, A. A., Roghani, A., Zamiri, M. J., and Zahedifar, M. (2004). Nutrition value of Gundelia tournefortii L in feeding of sheep. Journal of Science and Technology of Agriculture and Natural Resources, 8(1).
  27. Kelmanson, J. E., Jäger, A. K., and van Staden, J. (2000). Zulu medicinal plants with antibacterial activity. Journal of ethnopharmacology, 69(3): 241-246.
  28. Ko, C. W., Qu, J., Black, D. D., and Tso, P. (2020). Regulation of intestinal lipid metabolism: current concepts and relevance to disease. Nature Reviews Gastroenterology and Hepatology, 17(3): 169-183.
  29. Matthäus, B., and Özcan, M. M. (2011). Chemical evaluation of flower bud and oils of tumbleweed (Gundelia tourneforti L.) as a new potential nutrition sources. Journal of food biochemistry, 35(4): 1257-1266.
  30. Messora, M. R., Pereira, L. J., Foureaux, R., Oliveira, L. F., Sordi, C. G., Alves, A. J., ... and Furlaneto, F. A. (2016). Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats. Archives of Oral Biology, 66: 108-119.
  31. McCance, M. E., and Harrigan, W. F. (2000). Laboratory methods in food and dairy microbiology. Blackwell Science.
  32. Nelson, S. (2019). The antibacterial activity of essential oils from Tagetes erecta and Thuja occidentalis. Cantarus, 27: 29-33.
  33. Obeidat, M. (2011). Antimicrobial activity of some medicinal plants against multidrug resistant skin pathogens. Journal of Medicinal Plants Research, 5(16): 3856-3860.
  34. Qnais, E., Bseiso, Y., Wedyan, M., Al-Omari, M., and Alkhateeb, H. (2016). Chemical composition and antinociceptive effects of essential oil from aerial parts of Gundelia tournefortii L Asteraceae (Compositae) in rats. Tropical Journal of Pharmaceutical Research, 15(10): 2183-2190.
  35. Petit, P. R., Sauvaire, Y. D., Hillaire-Buys, D. M., Leconte, O. M., Baissac, Y. G., Ponsin, G. R., and Ribes, G. R. (1995). Steroid saponins from fenugreek seeds: extraction, purification, and pharmacological investigation on feeding behavior and plasma cholesterol. Steroids, 60(10): 674-680.
  36. Rafieian-Kopaei, M., Nasri, H., Nematbakhsh, M., Baradaran, A., Gheissari, A., Rouhi, H., ... and Ardalan, M. (2012). Erythropoietin ameliorates genetamicin-induced renal toxicity: A biochemical and histopathological study. Journal of nephropathology, 1(2): 109–116.
  37. Richardson, K., Schoen, M., French, B., Umscheid, C. A., Mitchell, M. D., Arnold, S. E., ... and Degoma, E. M. (2013). Statins and cognitive function: a systematic review. Annals of internal medicine, 159(10): 688-697.
  38. Sarper, F., Akaydin, G., Şimşek, I., and Yeşilada, E. (2009). An ethnobotanical field survey in the Haymana district of Ankara province in Turkey. Turkish Journal of Biology, 33(1): 79-88.
  39. (2001). Static Analysis 8th International Symposium, Paris, France.
  40. Sedigheh, A., Jamal, M. S., Mahbubeh, S., Somayeh, K., Mahmoud, R. K., Azadeh, A., and Fatemeh, S. (2011). Hypoglycaemic and hypolipidemic effects of pumpkin (Cucurbita pepo L.) on alloxan-induced diabetic rats. African Journal of Pharmacy and Pharmacology, 5(23): 2620-2626.
  41. Soltani, N., and Khayatkashani, M. (2015). Gundelia tournefortii as a green corrosion inhibitor for mild steel in HCl and H2SO4 solutions. International Journal of Electrochemical Science, 10(1): 46-62.
  42. Tietz, Y. (2005). Clinical Biochemistry 6thed, Mc Graw – Hill, Newyork, 825.
  43. Tiwari, A. (2017). Handbook of antimicrobial coatings. Elsevier.
  44. Yin, Y. N., Yu, Q. F., Fu, N., Liu, X. W., and Lu, F. G. (2010). Effects of four Bifidobacteria on obesity in high-fat diet induced rats. World journal of gastroenterology, 16(27): 3394–3401.
Statistics
Article View: 150
PDF Download: 130


Journal Management System. Powered by ejournalplus.com