Isolating and Identification Some Types of Bacteria that Cause Otitis Media and Detecting their Resistance Towards Antibiotics | ||
| Kirkuk Journal of Science | ||
| Article 3, Volume 18, Issue 4, December 2023, Pages 32-41 PDF (420.32 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.32894/kujss.2023.143295.1120 | ||
| Authors | ||
| suhail Najim Abdullah* 1; Shaymaa Naji Dahham2 | ||
| 1Department of Biology, College of Education for pure sciences/Tikrit university. Tikrit, Iraq | ||
| 2Department of Chemistry, College of Education for pure sciences/Tikrit university, Tikrit, Iraq | ||
| Abstract | ||
| One hundred ear swabs were collected from patients attending Hawija General Hospital, Azadi Teaching Hospital, and outpatient clinics in Kirkuk Governorate, for ages ranging between 2-45 years, During the period from October 27, 2022 to January 3, 2023. The results showed that 91 (91%) of samples have positive growth on MacConkey , mannitol and blood agar. While the remaining samples 9 (9%) had no growth. The samples that showed positive bacterial growth were identified as 139 isolates. The number of Gram positive isolates was 91, a rate of 65.5%, while the number of negative isolates was 48, a rate of 34.5%. The isolates were diagnosed using routine biochemical tests, and the diagnosis was confirmed by Vitek2 compact system device. The percentage of isolated bacterial species were Staphylococcus aureus by 34.53%, Staphylococcus epidermidis by 22.30%, Proteus mirabilis by 8.63%, Morganella morganii by 6.47%, Enterobacter sakazakii by 6.47%, Staphylococcus saprophyticus by 4.32%, E.coli by 3.60%, Klebsiella pneumonia by 3.60% And each of Kocuria kristinae, Proteus vulgaris, Comamonas testosterone by 2.88%, and Staphylococcus lentus by 1.44%. The results of the sensitivity test to ten types of antibiotics, namely Amikacin, Ceftazidime, Nitrofurantion, Imipenem, Azithromycin, Tetracycline, Cefotaxime, Tobramycin, Ofloxacin, Oxacillin, showed that all isolates were 100% resistant to the antibiotics Cetazidime and Cefotaxime, while they showed varying resistance to the rest of the antibiotics. Antibiotics: All isolates showed 100% sensitivity to Anti- imipenem. | ||
| Keywords | ||
| Otitis media infection; pathogenic bacteria; antibiotics | ||
| References | ||
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