H. Attallah, M., A. Al-Bayar, M. (2023). EFFECT OF CENTRIFUGATION AND MELATONIN HORMONE ON COOLED SEMEN CHARACTERISTICS OF IRAQI LOCAL ROOSTERS. Alustath, 21(2), 643-655. doi: 10.32649/ajas.2023.179771
M. H. Attallah; M. A. Al-Bayar. "EFFECT OF CENTRIFUGATION AND MELATONIN HORMONE ON COOLED SEMEN CHARACTERISTICS OF IRAQI LOCAL ROOSTERS". Alustath, 21, 2, 2023, 643-655. doi: 10.32649/ajas.2023.179771
H. Attallah, M., A. Al-Bayar, M. (2023). 'EFFECT OF CENTRIFUGATION AND MELATONIN HORMONE ON COOLED SEMEN CHARACTERISTICS OF IRAQI LOCAL ROOSTERS', Alustath, 21(2), pp. 643-655. doi: 10.32649/ajas.2023.179771
H. Attallah, M., A. Al-Bayar, M. EFFECT OF CENTRIFUGATION AND MELATONIN HORMONE ON COOLED SEMEN CHARACTERISTICS OF IRAQI LOCAL ROOSTERS. Alustath, 2023; 21(2): 643-655. doi: 10.32649/ajas.2023.179771

EFFECT OF CENTRIFUGATION AND MELATONIN HORMONE ON COOLED SEMEN CHARACTERISTICS OF IRAQI LOCAL ROOSTERS

ANBAR JOURNAL OF AGRICULTURAL SCIENCES
Article 35, Volume 21, Issue 2, November and December 2023, Pages 643-655    PDF (493.27 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2023.179771
Authors
M. H. Attallah; M. A. Al-Bayar
Department of Animal Production, College of Agriculture, University of Anbar, Ramadi, Iraq
Abstract
This experiment was conducted in the poultry fields of the Department of Animal Production / College of Agriculture, University of Anbar. During the period from From 1 December 2021 to 1 April 2022, by using 40 Iraqi native roosters at age 33 week. In order to study the effect of semen storage process after semen centrifugation and replacement of seminal plasma with Lake diluent 1960 intended for semen preservation with the addition of the hormone melatonin as an antioxidant on semen quality characteristics. This study was carried out by 200 birds (40 roosters and 160 chickens) raised in a hall divided into cages. It was divided into five treatments for each treatment three replicates according to the following division: T1: control treatment T2: add dilution in a ratio of 1:2 only T3: add diluent containing the hormone melatonin at a concentration (1 mMol. L‾¹) T4: use centrifugation with Adding the diluent in a ratio of 1:2 only T5: using centrifugation with the addition of a diluent containing the hormone melatonin at a concentration of (1 mMol. L‾¹), and for a period of 72 hours, the results showed a significant superiority (P<0.01) of the T3 treatment for the indicators of semen quality (Sperm motility, percentage of dead sperm, total abnormalities, acrosome, head, midsection and tail) at a 36-hour storage period compared to the control treatment and the rest of the treatments and during the different storage periods, while the rest of the treatment did not record any significant differences. We conclude from this study that it is possible to add the hormone melatonin at a concentration of (1 mMol. L‾¹) to semen preservation diluents in order to improve the characteristics of semen through the role of the hormone as an antioxidant, which reduces oxidative stress on sperms.
Keywords
Semen; Centrifugation; Melatonin hormone; Cryopreservation; Iraqi native roosters
References
  1. Alavi, S. M. H., and Cosson, J. (2006). Sperm motility in fishes: Effects of ions and osmotic pressure. Cell Biology International, Campinas, 30(1): 1-14.
  2. Al-Bayar, M. A., ALjugaifi, W. I., Mansoor, A. R., Nassif, O. M., and Atallah, M. H. (2019). Effect of supplementing extender with melatonin on iraqi native semen quality during cooling storage. Biochemical and Cellular Archives, 19(2): 2971-2975.
  3. Al-Daraji, H. J., B. T. O. Al-Tikriti, Kh. H. Hassan, and A. A. Al-Rawi. (2002). Using new techniques to estimate abnormalities in the semen of birds. Journal of Life Technology Research. 4(1): 47-64.
  4. Al-Daraji, H. J. (2007). Artificial Insemination in Poultry Birds - Ministry of Higher Education and Scientific Research - College of Agriculture - University of Baghdad.
  5. Atessahin, A., Bucak, M. N., Tuncer, P. B., and Kızıl, M. (2008). Effects of anti-oxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze–thawing process. Small Ruminant Research, 77(1): 38-44.
  6. Bejarano, I., Monllor, F., Marchena, A. M., Ortiz, A., Lozano, G., Jiménez, M. I., ... and Espino, J. (2014). Exogenous melatonin supplementation prevents oxidative stress‐evoked DNA damage in human spermatozoa. Journal of pineal research, 57(3): 333-339.
  7. Browne, R. K., Kaurova, S. A., Uteshev, V. K., Shishova, N. V., McGinnity, D., Figiel, C. R., ... and Cosson, J. (2015). Sperm motility of externally fertilizing fish and amphibians. Theriogenology, 83(1): 1-13.
  8. Burrows, W. H., and Quinn, J. P. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poultry Science, 16(1): 19-24.
  9. Cevik, M., Tuncer, P. B., Taşdemir, U., and Özgürtaş, T. (2007). Comparison of spermatological characteristics and biochemical seminal plasma parameters of normozoospermic and oligoasthenozoospermic bulls of two breeds. Turkish Journal of Veterinary and Animal Sciences, 31(6): 381-387.
  10. Douard, V., Hermier, D., Labbe, C., Magistrini, M., and Blesbois, E. (2005). Role of seminal plasma in damage to turkey spermatozoa during in vitro storage. Theriogenology, 63(1): 126-137.
  11. Druart, X., and de Graaf, S. (2018). Seminal plasma proteomes and sperm fertility. Animal reproduction science, 194: 33-40.
  12. Duncan, D. B. (1955). Multiple range and multiple F tests. biometrics, 11(1): 1-42.
  13. Dzyuba, V., and Cosson, J. (2014). Motility of fish spermatozoa: from external signaling to flagella response. Reproductive Biology, 14(3): 165-175.
  14. Gałęska, E., Wrzecińska, M., Kowalczyk, A., and Araujo, J. P. (2022). Reproductive Consequences of Electrolyte Disturbances in Domestic Animals. Biology, 11(7): 1006.
  15. García, J. J., López‐Pingarrón, L., Almeida‐Souza, P., Tres, A., Escudero, P., García‐Gil, F. A., ... and Bernal‐Pérez, M. (2014). Protective effects of melatonin in reducing oxidative stress and in preserving the fluidity of biological membranes: a review. Journal of pineal research, 56(3): 225-237.
  16. Harchegani, A. B., Irandoost, A., Mirnamniha, M., Rahmani, H., Tahmasbpour, E., and Shahriary, A. (2019). Possible mechanisms for the effects of calcium deficiency on male infertility. International journal of fertility and sterility, 12(4): 267.
  17. Hardeland, R., Cardinali, D. P., Srinivasan, V., Spence, D. W., Brown, G. M., and Pandi-Perumal, S. R. (2011). Melatonin—A pleiotropic, orchestrating regulator molecule. Progress in neurobiology, 93(3): 350-384.
  18. Henkel, R., Kierspel, E., Stalf, T., Mehnert, C., Menkveld, R., Tinneberg, H. R., ... and Kruger, T. F. (2005). Effect of reactive oxygen species produced by spermatozoa and leukocytes on sperm functions in non-leukocytospermic patients. Fertility and Sterility, 83(3): 635-642.
  19. Jang, H. Y., Kim, Y. H., Kim, B. W., Park, I. C., Cheong, H. T., Kim, J. T., ... and Yang, B. K. (2010). Ameliorative effects of melatonin against hydrogen peroxide‐induced oxidative stress on boar sperm characteristics and subsequent in vitro embryo development. Reproduction in domestic animals, 45(6): 943-950.
  20. Kumar, A., Sridharn, T. B., and Rao, K. A. (2019). Role of seminal plasma proteins in effective zygote formation-A success road to pregnancy. Protein and Peptide Letters, 26(4): 238-250.
  21. Lake, P. E. (1960). Studies on the dilution and storage of fowl semen. Reproduction, 1(1): 30-35.
  22. Long, J. A. (2014). Applied andrology in chickens and turkeys. Animal Andrology. Theories and Applications. Chenoweth PJ and Lorton SP, editors. CABI, Boston MA, USA, 197-225.
  23. López, A., García, J. A., Escames, G., Venegas, C., Ortiz, F., López, L. C., and Acuña‐Castroviejo, D. (2009). Melatonin protects the mitochondria from oxidative damage reducing oxygen consumption, membrane potential, and superoxide anion production. Journal of pineal research, 46(2): 188-198.
  24. Maitra, S. K., and Hasan, K. N. (2016). The role of melatonin as a hormone and an antioxidant in the control of fish reproduction. Frontiers in endocrinology, 7: 38.
  25. Maldjian, A., Cerolini, S., Surai, P., and Speake, B. K. (1998). The effect of vitamin E, green tea extracts and catechin on the in vitro storage of turkey spermatozoa at room temperature. Poultry and Avian Biology Reviews, 9(4): 143-151.
  26. Malik, M. I., Jamil, H., Qureshi, Z. I., Mehfooz, A., Rizvi, S. N. B., Ullah, S., ... and Khan, M. S. (2018). 32. Investigation on relationship of hormonal profile and biochemical constituents of seminal plasma with physical characteristics of Damani buck semen. Pure and Applied Biology (PAB), 7(2): 684-691.
  27. Malm, G., Haugen, T. B., Rylander, L., and Giwercman, A. (2017). Seasonal fluctuation in the secretion of the antioxidant melatonin is not associated with alterations in sperm DNA damage. Asian Journal of Andrology, 19(1): 52.
  28. Manchester, L. C., Coto‐Montes, A., Boga, J. A., Andersen, L. P. H., Zhou, Z., Galano, A., ... and Reiter, R. J. (2015). Melatonin: an ancient molecule that makes oxygen metabolically tolerable. Journal of pineal research, 59(4): 403-419.
  29. Meamar, M., Shahneh, A. Z., Zamiri, M. J., Zeinoaldini, S., Kohram, H., Hashemi, M. R., and Asghari, S. (2016). Preservation effects of melatonin on the quality and fertility of native Fars rooster semen during liquid storage. Czech Journal of Animal Science, 61(1): 42-48.
  30. Medeiros, C. M. O., Forell, F., Oliveira, A. T. D., and Rodrigues, J. L. (2002). Current status of sperm cryopreservation: why isn't it better?. Theriogenology, 57(1): 327-344.
  31. Miguel-Jiménez, S., Pina-Beltrán, B., Gimeno-Martos, S., Carvajal-Serna, M., Casao, A., and Pérez-Pe, R. (2021). NADPH Oxidase 5 and melatonin: Involvement in ram sperm capacitation. Frontiers in Cell and Developmental Biology, 9: 655794.
  32. Najafi, A., Adutwum, E., Yari, A., Salehi, E., Mikaeili, S., Dashtestani, F., ... and Asadi, E. (2018). Melatonin affects membrane integrity, intracellular reactive oxygen species, caspase3 activity and AKT phosphorylation in frozen thawed human sperm. Cell and tissue research, 372: 149-159.
  33. (1994). Nutrient requirements of domestic animals. Nutrient requirements of poultry.
  34. Ortiz, A., Espino, J., Bejarano, I., Lozano, G. M., Monllor, F., García, J. F., ... and Rodríguez, A. B. (2011). High endogenous melatonin concentrations enhance sperm quality and short‐term in vitro exposure to melatonin improves aspects of sperm motility. Journal of pineal research, 50(2): 132-139.
  35. Reiter, R. J., Mayo, J. C., Tan, D. X., Sainz, R. M., Alatorre‐Jimenez, M., and Qin, L. (2016). Melatonin as an antioxidant: under promises but over delivers. Journal of pineal research, 61(3): 253-278.
  36. Reiter, R. J., Tan, D. X., Manchester, L. C., Paredes, S. D., Mayo, J. C., and Sainz, R. M. (2009). Melatonin and reproduction revisited. Biology of reproduction, 81(3): 445-456.
  37. Santiago-Moreno, J., and Blesbois, E. (2020). Functional aspects of seminal plasma in bird reproduction. International Journal of Molecular Sciences, 21(16): 5664.
  38. (2012). Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.
  39. Smith, A. M. J., Bonato, M., Dzama, K., Malecki, I. A., and Cloete, S. W. P. (2018). Mineral profiling of ostrich (Struthio camelus) seminal plasma and its relationship with semen traits and collection day. Animal reproduction science, 193: 98-106.
  40. Suzen, S. (2018). Melatonin and its antiaging activity: new approaches and strategies for age-related disorders. Molecular Basis and Emerging Strategies for Anti-aging Interventions, 217-235.
  41. Tan, D. X., Manchester, L. C., Terron, M. P., Flores, L. J., and Reiter, R. J. (2007). One molecule, many derivatives: a never‐ending interaction of melatonin with reactive oxygen and nitrogen species?. Journal of pineal research, 42(1): 28-42.
  42. Zaniboni, L., and Cerolini, S. (2009). Liquid storage of turkey semen: Changes in quality parameters, lipid composition and susceptibility to induced in vitro peroxidation in control, n-3 fatty acids and alpha-tocopherol rich spermatozoa. Animal reproduction science, 112(1-2): 51-65.
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