J. Awad, A., A. Farhan, T. (2023). EFFICIENCY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF SOME PLANT EXTRACTS IN INHIBITING THE GROWTH OF FUSARIUM SOLANI THAT CAUSES COWPEA ROOT ROT DISEASE IN THE LABORATORY AND IN THE FIELD. Alustath, 21(2), 632-642. doi: 10.32649/ajas.2023.179770
A. J. Awad; T. A. Farhan. "EFFICIENCY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF SOME PLANT EXTRACTS IN INHIBITING THE GROWTH OF FUSARIUM SOLANI THAT CAUSES COWPEA ROOT ROT DISEASE IN THE LABORATORY AND IN THE FIELD". Alustath, 21, 2, 2023, 632-642. doi: 10.32649/ajas.2023.179770
J. Awad, A., A. Farhan, T. (2023). 'EFFICIENCY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF SOME PLANT EXTRACTS IN INHIBITING THE GROWTH OF FUSARIUM SOLANI THAT CAUSES COWPEA ROOT ROT DISEASE IN THE LABORATORY AND IN THE FIELD', Alustath, 21(2), pp. 632-642. doi: 10.32649/ajas.2023.179770
J. Awad, A., A. Farhan, T. EFFICIENCY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF SOME PLANT EXTRACTS IN INHIBITING THE GROWTH OF FUSARIUM SOLANI THAT CAUSES COWPEA ROOT ROT DISEASE IN THE LABORATORY AND IN THE FIELD. Alustath, 2023; 21(2): 632-642. doi: 10.32649/ajas.2023.179770

EFFICIENCY OF AQUEOUS AND ALCOHOLIC EXTRACTS OF SOME PLANT EXTRACTS IN INHIBITING THE GROWTH OF FUSARIUM SOLANI THAT CAUSES COWPEA ROOT ROT DISEASE IN THE LABORATORY AND IN THE FIELD

ANBAR JOURNAL OF AGRICULTURAL SCIENCES
Article 34, Volume 21, Issue 2, November and December 2023, Pages 632-642    PDF (610.08 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2023.179770
Authors
A. J. Awad; T. A. Farhan
Department of Plant Protection, College of Agriculture, University of Anbar, Ramadi, Iraq
Abstract
The results of phenotypic and molecular diagnosis using PCR technique showed that the main cause of cowpea root rot disease It is Fusarium solani that was isolated from the roots of cowpea plants infected with the disease, and the results of inhibition ability test  showed theaqueous extract of pomegranate peel, catnip and bitter melon fruits concentrations 10, 20, 30 ml. liter-1 The inefficiency of pomegranate peel extract and bitter melon fruit in all concentrations, while the aqueous extract of The catnip had a significant superiority in the percentage of inhibiting the pathogenic fungus F. solani on PDA culture medium, was 24.80%, 24.82%, 24.88%, respectively, compared to the control treatment contaminated with the pathogenic fungus F.solani, in which the percentage of inhibition was 0.00% The results of using alcoholic extract of pomegranate peels, catnip and bitter melon fruits at concentrations of 5, 10, and 15 mg were shown., as the extracts with all concentrations achieved a significant superiority over the control treatment, and the most effective of these extracts was the pomegranate peel extract at a concentration of 15 mg. ml-1 as the percentage inhibition was 77.30% compared to the control treatment contaminated with the pathogenic fungus F.solani, which had 0.00% inhibition And the results of using the alcoholic extract of pomegranate peels and catnip at a concentration of 10 mg. ml-1 were shown. when used in the field the efficiency of these two extracts in a significant increase in the percentage of germination of cowpea seeds and a significant decrease in the percentage and severity of infection percentage compared to the control treatment contaminated with the pathogenic fungus F.solani.
Keywords
Fusarium solani; Root rot; Cowpea; Plant extract
References
  1. Abyomi, Y. A., Ajibade, T. V., Samuel, O. F., and Adudeen, S. B. (2008). Growth and yield reponses of cowpea (Vigna unguiculata L. Walp) genotypes to nitrogen fertilizer (NPK) application in the Southern Guinea Savanna zone of Nigeria. Asian Journal of Plant Sciences, 7(2): 170-176.
  2. Abou El Nour, M. M., Mona, E. A. D. S., and Wadi, J. M. (2020). Suppressive effect of compost/pomegranate peel tea combination against Fusarium oxysporum f. sp. lupini, and Rhizoctonia solani as an alternative synthetic fungicide. The Egyptian Journal of Experimental Biology, 16(1): 13-25.
  3. Al-Kaif, M. A. I. K. (2015). Isolation and identification of the causes of rotting roots and bases of bean stalks in Babylon Governorate and their resistance to some biological factors and plant extracts. Master Thesis. Technical College. Al-Musayyib.
  4. Annual Statistical Collection. (2020). Agricultural Statistics Directorate. central Statistical Organization. Ministry of Planning. Iraq.
  5. Barnett, H. L., and Hunter, B. B. (1998). Illustrated genera of imperfect fungi. The American phytopathological society. US Department of Agriculture, Agricultural Research Service, Washington State University, Pullman. APS Press. USA. St. Paul, Minnesota USA. 218p.‏
  6. Boukar, O., Belko, N., Chamarthi, S., Togola, A., Batieno, J., Owusu, E., ... and Fatokun, C. (2019). Cowpea (Vigna unguiculata): Genetics, genomics and breeding. Plant Breeding, 138(4): 415-424.
  7. Chehri, K., Salleh, B., Yli-Mattila, T., Reddy, K. R. N., and Abbasi, S. (2011). Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah province, Iran. Saudi journal of biological sciences, 18(4): 341-351.
  8. Dewan, M. M. (1989). Identify and frequency of occurrence of fungi in root of wheat and ryegrass and their effect on take – all and hostgrowth. Ph.D. Thesis. Univ. West Australia. 210.
  9. Elshafie, H. S., Caputo, L., De Martino, L., Sakr, S. H., De Feo, V., and Camele, I. (2021). Study of bio-pharmaceutical and antimicrobial properties of pomegranate (Punica granatum L.) leathery exocarp extract. Plants, 10(1): 153.
  10. Giménez-Santamarina, S., Llorens-Molina, J. A., Sempere-Ferre, F., Santamarina, C., Roselló, J., and Santamarina, M. P. (2022). Chemical composition of essential oils of three Mentha species and their antifungal activity against selected phytopathogenic and post-harvest fungi. All Life, 15(1): 64-73.
  11. Gülçın, İ., Oktay, M., Kıreçcı, E., and Küfrevıoǧlu, Ö. İ. (2003). Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food chemistry, 83(3): 371-382.
  12. Hussein, M. A. M., and Hassan, S. H. A. (2022). Possible sustainable management of onion Botrytis brown stain disease by some plants aqueous extract. SVU-International Journal of Agricultural Sciences, 4(2), 41-52.
  13. Moghaddam, M., Pourbaige, M., Tabar, H. K., Farhadi, N., and Hosseini, S. M. A. (2013). Composition and antifungal activity of peppermint (Mentha piperita) essential oil from Iran. Journal of Essential Oil Bearing Plants, 16(4): 506-512.
  14. Neeraj, V. S., and Verma, S. (2010). Alternaria diseases of vegetable crops and new approaches for its control. Asian Journal of Experimental Biological Sciences, 1(3): 681-692.
  15. Rongai, D., Pulcini, P., Pesce, B., and Milano, F. (2017). Antifungal activity of pomegranate peel extract against fusarium wilt of tomato. European Journal of Plant Pathology, 147: 229-238.
  16. Seema, M. S. S. E., Sreenivas, S. S., Rekha, N. D., and Devaki, N. S. (2011). In vitro studies of some plant extracts against Rhizoctonia solani Kuhn infecting FCV tobacco in Karnataka Light Soil, Karnataka, India. Journal of Agricultural technology, 7(5): 1321-1329.
  17. Soliman, S. A., Hafez, E. E., Al-Kolaibe, A. M., Abdel Razik, E. S. S., Abd-Ellatif, S., Ibrahim, A. A., ... and Elshafie, H. S. (2022). Biochemical Characterization, Antifungal Activity, and Relative Gene Expression of Two Mentha Essential Oils Controlling Fusarium oxysporum, the Causal Agent of Lycopersicon esculentum Root Rot. Plants, 11(2): 189.
  18. Thanaboripat, D., Suvathi, Y., Srilohasin, P., Sripakdee, S., Patthanawanitchai, O., and Charoensettasilp, S. (2007). Inhibitory effect of essential oils on the growth of Aspergillus flavus. Current Applied Science and Technology, 7(1): 1-7.
  19. Thobunluepop, P., Jatisatienr, C., Pawelzik, E., and Vearasilp, S. (2008). In vitro screening of the antifungal activity of plant extracts as fungicides against rice seed borne fungi. In Asia Pacific Symposium on Assuring Quality and Safety of Agri-Foods, 837: 223-228.
  20. Zughayer, F. S., A. A. Obaid., and A. H. Rashid. (2010). Estimation of the incidence of tomato seed rot disease and the death of its seedlings for some desert farms of Karbala and the possibility of chemical control. Al-Furat Journal of Agricultural Sciences, 2(1): 104-114.
Statistics
Article View: 107
PDF Download: 64


Journal Management System. Powered by ejournalplus.com