Spectrophotometric Determination of Isoniazid in Pharmaceutical Preparations Using Oxidative Coupling Reaction with 2,4-dinitrophenylhydrazine | ||
| Rafidain Journal of Science | ||
| Article 16, Volume 32, Issue 1, March 2023, Pages 149-160 PDF (664.86 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/rjs.2023.178227 | ||
| Authors | ||
| Shaimaa M. Nayif* 1; Dawood H. Mohammed2 | ||
| 1Department of Biochemistry/ College of Medicine/ University of Mosul | ||
| 2Department of Chemistry/ College of Education for Girls/ University of Mosul | ||
| Abstract | ||
| A spectrophotometric method is proposed to determine isoniazid using oxidative coupling reaction. The proposed method depends on adding the oxidizing agent potassium periodate to the reagent solution 2,4-dinitrophenylhydrazine. Different optimum conditions are studied and explained based on various experiments and tests. The wavelength is (406) nm. Beer's law within the concentration (0.2-40) µg/ml, and with a molar absorptivity value of 0.2482 × 16104"> l.mol-1.cm-1, while the recovery was (98.46%), and the relative standard deviation did not exceed 1.34. The method was successfully applied for the determination of isoniazid in its pharmaceutical preparations (medicine tablets). | ||
| Keywords | ||
| Spectrophotometric method; isoniazid; pharmaceutical preparations; 2; 4-dinitrophenylhydrazine | ||
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