Spectrophotometric Determination of Sulphacetamide Sodium Using 2,4-Dinitrophenylhydrazine as Coupling Reagent | ||
| College Of Basic Education Research Journal | ||
| Article 31, Volume 19, Issue 1, March 2023, Pages 741-752 PDF (676.91 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/berj.2023.178135 | ||
| Authors | ||
| Thukaa M. Abdulrazzaq M . Abdulrazzaq* ; Saad H Sultan* | ||
| Department of Chemistry, College of Science ,University of Mosul , Mosul , Iraq | ||
| Abstract | ||
| A Sensitive, rapid and selective spectrophotometric method was developed for the determination of sulfacetamide sodium in an aqueous solution. The method is based on the oxidative coupling reaction with 2,4-dinitophenylhydrazine in the basic medium in the presence of potassium periodate to produce an intense orange colour product, soluble in water, stable and gives maximum absorbance at 484 nm. The linearity of Beer's law was in the range of 2.5-75 μg/ml of sulfacetamide. The molar absorptivity, Sandellʼs sensitivity, and detection limit were 3202.92 L/mol.cm, 0.07936 µg/cm2 and 0.50476 μg/ml respectively. This method is applied successfully to the determination of sulfacetamide in pharmaceutical preparation (eye drops and ointment). | ||
| Keywords | ||
| Sulphacetamide sodium; oxidative coupling reaction; 2; 4- dinitrophenylhydrazine; spectrophotometry | ||
|
Statistics Article View: 166 PDF Download: 220 |
||