الخفاجی, ., Al- Mudhafar, A., alkhafaji, B. (2023). The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR). Alustath, 13(1), 123-132. doi: 10.33794/qjas.2023.138240.1125
باسم الخفاجی; Adnan Wahhab Habeeb Al- Mudhafar; basim alkhafaji. "The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR)". Alustath, 13, 1, 2023, 123-132. doi: 10.33794/qjas.2023.138240.1125
الخفاجی, ., Al- Mudhafar, A., alkhafaji, B. (2023). 'The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR)', Alustath, 13(1), pp. 123-132. doi: 10.33794/qjas.2023.138240.1125
الخفاجی, ., Al- Mudhafar, A., alkhafaji, B. The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR). Alustath, 2023; 13(1): 123-132. doi: 10.33794/qjas.2023.138240.1125

The Use of Sugar Cane Waste (Molasses) in the Production of Lactic Acid by L. paracasei CAU 9836 and its Identification by Infrared Spectrum (FT.IR)

Al-Qadisiyah Journal For Agriculture Sciences
Article 13, Volume 13, Issue 1, June 2023, Pages 123-132    PDF (1.03 M)
Document Type: Research Paper
DOI: 10.33794/qjas.2023.138240.1125
Authors
باسم الخفاجی1; Adnan Wahhab Habeeb Al- Mudhafar2; basim alkhafaji* 3
1food science , agriculture college , university of kufa
2Food Science Department - Faculty Of Agriculture-University of Kufa Specialty: food science and Biotechnology
3قسم علوم الاغذیة , کلیة الزراعة و جامعة الکوفة , العراق
Abstract
A total of twelve bacterial isolates from different samples of yoghurt and milk purchased from local markets in Diwaniyah Governorate. The preliminary screening was carried out all bacterial isolates by gram staining. The six isolates were selected based on the intensity of colour variation in the fermentation medium and were identified according to phenotypic, microscopic, and biochemical examinations, which included twelve isolates of Lactobacillus spp.  Vitek II Compact instrument was used with all isolates to confirm the initial identification. The secondary screening was performed for isolates of Lactobacillus spp.species. Given the amount of the produced lactic acid, the L.casei strain 1859 had the highest lactic acid production, which was 64.32 g/L. The 16S rRNA test allowed the identification of this isolate.The results showed that the best conditions for acid production were 62.76 mg/L and the L.casei strain 9836 grown on the alternative medium containing 50 % whey, 7% molasses, and pH 6.9, incubated on an orbital shaker at 38°C for 24 hours, and agitated at 250 rpm.FTIR techniques were used to identify the nucleus of the hydrogen atom (proton) 1H and the carbon atoms 13C in the presence of standard lactic acid for comparison. The results revealed the peaks and bands of the produced acid were some similar to that of the standard acid.
Keywords
isolation,,; ,،,؛biochemical,,; ,،,؛fermentation,,; ,،,؛diagnosis
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