The genetic diagnosis of the bacteria isolated from the agricultural soil sustained by the polymerase chain reaction technique qPCR | ||
| ANBAR JOURNAL OF AGRICULTURAL SCIENCES | ||
| Articles in Press, Accepted Manuscript, Available Online from 23 February 2023 | ||
| Document Type: Research Paper | ||
| DOI: 10.32649/ajas.2023.177261 | ||
| Author | ||
| Saja Khaleed Al-any* | ||
| کلیة الزراعه جامعة الانبار | ||
| Abstract | ||
| Thefieldpartinvolvedinvestigationsinsustainableagriculturalfields to look for the presence and spread of bacterial biofertilizers by bringing soil samplesfrom14 sitesindifferentagriculturalareas, including Abu GhraibandAnbar. Thesamplesweretakenfromrhizosphere areaofplantswhichexist intheagriculturalfields.Themeasurements and tests of samples conducted in the laboratories of theCollegeofAgricultureincluded isolating biofertilizersandtesting theefficiencyofisolatesthatinhibitatmosphericnitrogenandsolvents for phosphorous compounds, andbacteriawereisolatedanddiagnosed from rhizospheresoilsof differentplantswhichwerebroughtfromdifferentagriculturalareas,as74bacterialisolateswereobtainedaccordingtothecontrastofthephenotypiccharacteristicsofthedevelopingcoloniesonthechosen medium and biochemical and microscopic traits, the results of isolation and diagnosis showed that 43 isolates were obtained from the 14 different sites, and that the resultsofthequantitative polymeraseinteraction(qPCR)examinationshowedthatitconsider diagnosticresultsusingthequantitativepolymeraseinteractiontechniqueafterextractDNAfrombacterial isolates which isolated from the soil, as the results of thefirst group(10)isolatesofPseudomonas butida bacteria showed when the gene (GltAF,GltAR)amplified, andtheresultingbundlesofmolecular size were 288 base pairsfor Pseudomonasbutidabacteriaafter confirming thediagnosisofthe bacteriausingthemolecularmethodandbiological,microscopicand phenotypic tests and that this match confirms the accuracy of theclassificationofPseudomonas butida bacteria and thesecond group(10)IsolatesPseudomonas butida bacteria results showed when the gene amplified(pRoDF, Rp o DR)andtheresultingbundles with a molecular sizeof478base pairs of Bacillusmegateriumand confirmed it belongs to Bacillus megaterium, whilethethirdgroup (10)isolatesofbacteriaupongene amplification (N i f HF, N i f HR) theresultingbundleswithamolecular size of 371basepairsbelong to Azotobacter chroococcum bacteria and confirmed theirreturn to bacteria after confirming the diagnosis of the bacteria Azotobacter chroococcum. As for the fourth group (14) isolates when the gene was amplified (16sAZ F and 16sAZ R) and the resulting bundles with molecular size 646 base pairs for Azospirillu Lipoferum bacteria and confirmed its belongs to Azospirillum Lipoferum bacteria, while the fifth group did not show any type of nitrogen-inhibit bacteria or solvent for phosphate in those bundles after amplification, based on the SrDNA 71 genotype sequence. | ||
| Keywords | ||
| Bactiria,,; ,،,؛Biofertilizers,,; ,،,؛Dna,,; ,،,؛Pcr algene | ||
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