Biodegradation of Food Waste by Mesophilic and Thermophilic Microorganisms in Duhok city | ||
Kirkuk Journal of Science | ||
Article 4, Volume 17, Issue 4, December 2022, Pages 34-41 PDF (196.8 K) | ||
Document Type: Research Paper | ||
DOI: 10.32894/kujss.2022.136291.1079 | ||
Authors | ||
Adel Omar Ahmad1; Hussein Ali Sadeq2; Yousif Abdullah Albany* 3; Mohammad Ismail Al-Berfkani4 | ||
1Medical Laboratory Technology, College of Health and Medical Techniques, Duhok Polytechnic University. Duhok, Iraq | ||
2Medical Laboratory Technologyو College of Health and Medical Techniques, Duhok Polytechnic University, Duhok, Iraq. | ||
3Medical Laboratory Technology, College of Health and Medical Techniques, Duhok Polytechnic University, Duhok, Iraq | ||
4Medical Laboratory Technology, College of Health and Medical Techniques, Duhok Polytechnic University, Duhok, Iraq. | ||
Abstract | ||
Garbage kitchen waste has been considered as one of the most difficult global issues with negative effect on the environment and health. It contains high amounts of cellulose and other organic waste which become ideal environments for the growth of pathogenic microbes and their toxic products which may reach agricultural land and water system. As a compromise, certain microbes are implemented under certain conditions to reduce and manage food waste. In the present study microorganism isolated from garbage kitchen waste collected in Duhok city. one thermophilic bacterium, two mesophilic bacteria, and fungus. All isolates were identified to the genus level and verified by morphological and biochemical characteristics. The findings show that two mesophilic bacteria were fungus from the genera Mucor and Rhizopus, one was a bacterium from the family Staphylococci, and one was a thermophilic Bacillus species. The enzyme activities of the isolates were estimated and revealed that Bacillus spp. has cellulase, protease and catalase activity while Staphylococcus spp. has only protease and catalase activity. Mucor and Rhizopus spp. have only cellulase and catalase activity. To estimate the level of garbage degradation. The level of garbage degradation was estimated by tacking the weight of the sample before and after degradation as well as detecting the content of protein and carbohydrate by using Folin Lowry and di-nitro salicyclic acid, phenol sulphuric acid method. The comparative study showed that both mesophilic (Mucor) and thermophilic microorganisms (Bacillus) showed the best degrader compared with Staphylococcus and Rhizopus. | ||
Keywords | ||
KEYWORDS: Biodegradation; Mesophilic; Thermophilic; Enzyme activity; Food waste | ||
References | ||
[1] V.P. Upadhyay, M.R. Prasad, A. Shrivastav, and K. Singh. Ecotools for urban waste management in india. The Journal of Human Ecology, 18(4):253–269, 2005.
[2] R. Ullah, R.N. Malik, and A. Qadir. Assessment of groundwater contamination in an industrial city, sialkot, pakistan. African Journal of Environmental Science and Technology, 3(12):429–446, 2009.
[3] R. Qadri and M.A. Faiq. Ionospheric fof2 variability at equatorial and low latitudes during high, moderate and low solar activity. Indian Journal of Radio Space Physics, 40:124–129, 2011.
[4] Y. Albany. Isolation and identification of coliforms in drinking water of duhok province by using membrane with vitek compact. In Research Innovation amid Global Pandemic, pages 145–150. International Engineering Conference, 2021.
[5] A. J. Al-barwary, H.S. Saadi, W. M. Salih, and A. J. Ahmed. Gastroduodenal endoscopic findings and seroprevalence of helicobacter pylori among suspected individuals. Kirkuk University Journal-Scientific Studies, 17(3):1–6, 2022.
[6] K. Schanes, K. Dobernig, and B. Gozet. Food waste ¨ matters-a systematic review of household food waste practices and their policy implications. Journal of cleaner production, 182:978–991, 2018.
[7] A. Ahmed, A. Hussain, S. Thahrani, S. Ahmed, A.Q. Khoso, and B. Soomro. Municipal solid waste management and waste to energy in karachi pakistan. Engineering Proceedings, 12(1):19, 2021.
[8] T. Nishino, T. Nakayama, H. Hemmi, T. Shimoyama, S. Yamashita, M. Akai, T. Kanagawa, and K. Hoshi. Acidulocomposting, an accelerated composting process of garbage under thermoacidophilic conditions for prolonged periods. j. environ. Biotechnol, 3(1):33–36, 2003.
[9] K. Palaniveloo, M.A. Amran, N.A. Norhashim, N. Mohamad-Fauzi, F. Peng-Hui, L. Hui-Wen, Y. Kai-Lin, L. Jiale, M.G. Chian-Yee, L. Jing-Yi, and B. Gunasekaran. Food waste composting and microbial community structure profiling. Processes, 8(6):723, 2020.
[10] Hal S. Alper Beena C. Lad, Sarah M. Coleman. Microbial valorization of underutilized and nonconventional waste streams. Journal of Industrial Microbiology and Biotechnology, 49(2):056, 2022.
[11] L.S. Dilkes-Hoffman. Exploring the role of biodegradable plastics. The University of Queensland School of Chemical Engineering, Australia, 2020.
[12] Y. A. AlBany, M. I. Al-Berfkani, and M. S. Assaf. Phage therapy against biofilm of multidrug-resistant klebsiella pneumoniae isolated from zakho hospital samples. Polytechnic Journal, 9(1):17–22, 2019.
[13] PP. Rath, K. Das, and S. Pattanaik. Microbialactivity during composting and plant growth impact: A review. Journal of Pure and Applied Microbiology, 16(1):63–73, 2022.
[14] M. Rastogi, M. Nandal, and B. Khosla. Microbes as vital additives for solid waste composting. Heliyon, 6(2):e03343, 2020.
[15] S.A. Boers, R. Jansen, and J.P. Hays. Understanding and overcoming the pitfalls and biases of next-generation equencing (ngs) methods for use in the routine clinical microbiological diagnostic laboratory. European Journal of Clinical Microbiology Infectious Diseases, 38(6):1059–1070, 2019.
[16] L.R. Cooperband. Composting: art and science of organic waste conversion to a valuable soil resource. Laboratory medicine, 31(5):283–290, 2000.
[17] K. Azim, B. Soudi, S. Boukhari, C. Perissol, S. Roussos, and I. Thami Alami. Composting parameters and compost quality: a literature review. Organic agriculture, 8(2):141– 158, 2018.
[18] C.M. Mehta, U. Palni, I.H. Franke-Whittle, and A.K. Sharma. Compost: its role, mechanism and impact on reducing soil-borne plant diseases. Waste management, 34(3):607–622, 2014.
[19] Y.J. Park, H. Tsuno, T. Hidaka, and J.H. Cheon. Evaluation of operational parameters in thermophilic acid fermentation of kitchen waste. Journal of Material Cycles and Waste Management, 10(1):46–52, 2008.
[20] I.Y. Tafinta, K. Shehu, H. Abdulganiyyu, A.M. Rabe, and A. Usman. Isolation and identification of fungi associated with the spoilage of sweet orange (citrus sinensis) fruits in sokoto state. International Journal of Biotechnology and Food Science, 1(1):13–22, 2013.
[21] A. Oyeleke and S.B. Manga. Essential of Laboratory Practice. Minna, Niger state, Nigeria, 3ed edition, 2008.
[22] B.C. Adebayo-Tayo, N. Odu, C.U. Esen, and T.O. Okonko. Microorganisms associated with spoilage of stored vegetables in uyo metropolis, akwa ibom state, nigeria. Natural Sciences, 10(3):23–32, 2012.
[23] D. H. Bergey. Bergey’s manual of determinative bacteriology. Lippincott Williams Wilkins, 9th edition, 1994.
[24] N.D.Q. Ribeiro, T.P. Souza, L.M.A.S. Costa, C.P.D. Castro, and E.S. Dias. Microbial additives in the composting process. Ciencia e Agrotecnologia ˆ , 41:159–168, 2017.
[25] B.A. Samsu, R.M. Nafis, H.L.S. Abd, A.M. Najib, A.A. Suraini, R.N. Abduland S.U.K. Md, H.M. Ali, S. Kenji, and S. Yoshihito. Isolation and characterization of thermophilic cellulase-producing bacteria from empty fruit bunches-palm oil mill effluent compost. American Journal of Applied Sciences, 7(1):56–62, 2010.
[26] L.R. Lynd, P.J. Weimer, W.H. Van Zyl, and I.S. Pretorius. Microbial cellulose utilization: fundamentals and biotechnology. Microbiology and molecular biology reviews, 66(3):506–577, 2002.
[27] B.S. Mienda, A. Yahya, I.A. Galadima, and M.S. Shamsir. An overview of microbial proteases for industrial applications. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5(1):388–396, 2014.
[28] A. Razzaq, S. Shamsi, A. Ali, Q. Ali, M. Sajjad, A. Malik, and M. Ashraf. Microbial proteases applications. Frontiers in bioengineering and biotechnology, 7:110, 2019.
[29] G.M. Zeng, H.L. Huang, D.L. Huang, X.Z. Yuan, R.Q. Jiang, M. Yu, H.Y. Yu, J.C. Zhang, R.Y. Wang, and X.L. Liu. Effect of inoculating white-rot fungus during different phases on the compost maturity of agricultural wastes. Process Biochemistry, 44(4):396–400, 2009.
[30] V.R. de Figueirecirc, E.T. Martos, F. Gonccedil, W.P. Maciel, R. da Silva, D.L. Rinker, and E.S. Dias. Microbial inoculation during composting improves productivity of sun mushroom (agaricus subrufescens peck). African Journal of Microbiology Research, 7(35):4430– 4434, 2013.
[31] M.D. Vargas-Garcia, F.F. Suarez-Estrella, M.J. L ´ opez, ´ and J. Moreno. Influence of microbial inoculation and co-composting material on the evolution of humic-like substances during composting of horticultural wastes. Process Biochemistry, 41(6):1438–1443, 2006.
[32] C. Sanchez. Lignocellulosic residues: biodegradation ´ and bioconversion by fungi. Biotechnology advances, 27(2):185–194, 2009.
[33] E.M. Machado, R.M. Rodriguez-Jasso, J.A. Teixeira, and S.I. Mussatto. Growth of fungal strains on coffee industry residues with removal of polyphenolic compounds. Biochemical Engineering Journal, 60:87–90, 2012. [34] I.A. Adeyemo, O.E. Adetoyi, M.O. Oni, M.J. Ayodele, and A.B. Olayemi. Studies on degradation of waste pa[1]pers using microflora/microbial consortia isolated from refuse dumpsites in ilorin metropolis. International Journal of Biotechnology and Food Science, 1(1):13–22, 2013. | ||
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