THE USE OF AZOTOBACTER AND AZOSPIRILLUM AS A CATALYST FOR COEXISTENCE OF ZUCCHINI SQUASH WITH SALINITY | ||
| ANBAR JOURNAL OF AGRICULTURAL SCIENCES | ||
| Article 6, Volume 19, Issue 1, June 2021, Pages 69-83 PDF (1.24 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.32649/ajas.2021.175974 | ||
| Authors | ||
| R. A. AL-Hadithi; A. A. Al-Rawi* | ||
| University of Anbar - College of Agriculture | ||
| Abstract | ||
| Chroococcum and A. brasilense bacteria were isolated and identified from the root and the surrounding area of Arundo donax andCladium marisas plants growing in different areas in Ramadi city in order to be used in pollinating the seeds of zucchini squash plant. A pot experiment was carried out in the spring season on 16 ⁄ 3 ⁄ 2019 in one of the fields of the College of Agriculture - University of Anbar, by following the design of complete randomized sectors RCBD. It included two factors of salinity of irrigation water and four levels of S0, S1, S2 and S3 (1.3, 3, 5 and 7 dSm-1) and the factor of adding the bacterial isolates of Azotobacter L1 and Azospirillum L2 and their two mixtures L3, and without adding the L0 inoculum in order to study the effect of bacterial pollination on some growth characteristics of squash plants irrigated with different salinity water levels. The results showed a decrease in plant height, number of leaves and dry weight of the shoots from 53.48 cm, 33.00 leaf-1, 25.97 gm-1 at the S0 level, to 26.95 cm, 14.08 g-leaf-1, and 12.41 g-leaf-1 at the S3 level. The treatment of the two isolates mixture L3 achieved a significant increase in plant height, number of leaves and dry weight, and it was 55.53 cm, 44.00 leaf-1 and 28.33 g-leaf-1 compared with the comparison treatment at the S0 level. The salinity of irrigation water caused an increase in the nitrogen concentration in the vegetative parts of the plant from 1.77% to 2.07%, and caused a decrease in the concentration of phosphorus and potassium from 0.63 and 2.37% to 0.21 and 0.56%. The concentration of nitrogen, phosphorus and potassium significantly increased in the vegetative parts of the plant when the bacterial inoculation treatments were obtained, and the treatment L3 recorded the highest values and reached 2.75% N at the level of salinity of S3 and gave the highest value for the concentration of phosphorus and potassium, which were 0.75 and 2.87%, respectively, at the S0 level. | ||
| References | ||
|
1. Abbas, K. F. (2010). Effect of salinity on seed germination and growth of tomato seedlings (Lycopersicon esculentum). University of Thi-Qar Journal of Science, 2(3): 78-92.
2. Al-Hadithi, Y. K. H. (2011). The use of some organic waste, lime, and gypsum in salt water treatment and its effect on some soil characteristics and soybean growth.Doctoral dissertation, University of Anbar, pp. 52.
3. Al-Hamdani, F. M. A. (2000). The effect of interaction between salinity of irrigation water and fertilizer Phosphates on some soil properties and wheat yield. Doctoral dissertation, University of Anbar, pp. 42.
4. Ali, N. A. S., Al-Jumaili, A. A., and Rahi, H. S. (2014). Soil fertility. The Arab Society Library and the Scientific Books House for printing, publishing and distribution. College of Agriculture - University of Baghdad, pp. 24-26.
5. Aragüés, R., Puy, J., & Isidoro, D. (2004). Vegetative growth response of young olive trees (Olea europaea L., cv. Arbequina) to soil salinity and waterlogging. Plant and Soil, 258(1): 69-80.
6. Alrawi, K. M., and Khalaf, A. M. (1980). Design and analysis of experiments Agricultural. Ministry of Higher Education and Scientific and Research. College of Agriculture and Forestry. University Mosul. pp. 33-34.
7. Argo, B. (2003). Understanding pH management and plant nutrition. Journal of the International Phalaenopsis Alliance, 13 (4): 734-783.
8. Al-Uqaili, J. K., A. K. A. Jarallah, B. H. A. Al-Ameri, and F. A. Kredi. (2007). Effect saline drainage water on wheat growth and on soil salinity. Iraqi Journal of Science, 7 (special issue): 157-166.
9. Abass, D. K. (2015). Effect of irrigation water quality and organic and mineral fertilization on the availability of some nutrient elements and cabbage yield (Brassica oleracea var. capitate L.). Euphrates Journal of Agriculture Science, 7(4): 235-247.
10. Bashan, Y., & De-Bashan, L. E. (2010). How the plant growth-promoting bacterium Azospirillum promotes plant growth—a critical assessment. Advances in Agronomy, 108, 77-136.
11. Becking, JH. (1981). The Family Azotobacteraceae. In: Starr, M.P., Stolp, H., Trüper, H.G., Balows, A., Schlegel, H.G. (eds) The Prokaryotes. Springer, Berlin, Heidelberg.
12. Bernstein, N., Silk, W. K., & Läuchli, A. (1993). Growth and development of sorghum leaves under conditions of NaCl stress. Planta, 191(4): 433-439.
13. Boddey, R. M., Baldani, V. L., Baldani, J. I., and Döbereiner, J. (1986). Effect of inoculation of Azospirillum spp. on nitrogen accumulation by field-grownwheat. PlantandSoil, 95(1):109-121.
14. Chapman, H. D., & Pratt, P. F. (1962). Methods of analysis for soils, plants and waters. Soil Science, 93(1): 68.
15. Cuin, T. A., Tian, Y., Betts, S. A., Chalmandrier, R., & Shabala, S. (2009). Ionic relations and osmotic adjustment in durum and bread wheat under saline conditions. Functional Plant Biology, 36(12): 1110-1119.
16. Forum for Nuclear Cooperation in Asia. (2011). Public Opinion Surveys on Nuclear Energy in Seven FNCA Countries. In Forum for Nuclear Cooperation in Asia (FNCA), Public Information Project Leaders Meeting (pp. 3-18). Hanoi, Vietnam: Forum for Nuclear Cooperation in Asia.
17. Glick, B. R., Penrose, D. M., & Li, J. (1998). A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. Journal of theoretical biology, 190(1): 63-68.
18. Gupta, B., & Huang, B. (2014). Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. International journal of genomics, 2014.
19. Hafeez, F. Y., Yasmin, S., Ariani, D., Renseigné, N., Zafar, Y., & Malik, K. A. (2006). Plant growth-promoting bacteria as biofertilizer. Agronomy for sustainable development, 26(2): 143-150.
20. Hamdia , M. A . E. S., Shaddad, M. A. K., and Doaa , M. M. (2004). Mechanisms of salt tolerance and interactive effects of Azospirillum brasilense inoculation on maize cultivars grown under salt stress conditions. Plant Growth Regulation, 44(2): 165-174.
21. Hussein, H. A., Hawi, N. H., & Atallah, H. S. (2021). Effect of two types of foliar fertilizers on the growth and yield of summer squash (Cucurbita pepo L) grown in unheated greenhouses. Euphrates Journal of Agriculture Science, 13 (1):90-97.
22. Jackson, M. L. (1958). Soil chemical analysis prentice Hall. Inc., Englewood Cliffs, NJ, 498, 183-204.
23. Khan, H. A., Siddique, K. H., & Colmer, T. D. (2017). Vegetative and reproductive growth of salt-stressed chickpea are carbon-limited: sucrose infusion at the reproductive stage improves salt tolerance. Journal of Experimental Botany, 68(8): 2001-2011.
24. Kausar, A., & Gull, M. (2014). Nutrients uptake and growth analysis of four sorghum (Sorghum bicolor L.) genotypes exposed to salt stress. Pensee Journal, 76(4): 102-110.
25. Khan, Z., Tiyagi, S. A., Mahmood, I., and Rizvi, R. (2012). Effects of N fertilisation, organic matter, and biofertilisers on the growth and yield of chilli in relation to management of plant-parasitic nematodes. Turkish Journal of Botany, 36(1): 73-81.
26. Myint, A. K., Yamakawa, T., Kajihara, Y., & Zenmyo, T. (2010). Application of different organic and mineral fertilizers on the growth, yield and nutrient accumulation of rice in a Japanese ordinary paddy field. Science World Journal, 5(2): 48-54.
27. Olsen, S. R., & Sommers, L. E. (1982). Phosphorus. p. 403–430. AL Page et al.(ed.) Methods of soil analysis. Part 2. Agron. Monogr. 9. ASA and SSSA, Madison, WI. Phosphorus. p. 403–430. In AL Page et al.(ed.) Methods of soil analysis. Part 2. 2nd ed. Agron. Monogr. 9. ASA and SSSA, Madison, WI.
28. Richardson, A. E., Barea, J. M., McNeill, A. M., & Prigent-Combaret, C. (2009). Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and soil, 321(1): 305-339.
29. Ramakrishnan, K., and Selvakumar, G. (2012). Effect of biofertilizers on enhancement of growth and yield on Tomato (Lycopersicum esculentum Mill.). International Journal of Research in Botany, 2(4): 20-23.
30. Sarkar, D., Sankar, A., Devika, O. S., Singh, S., Parihar, M., Rakshit, A., and Datta, R. (2021). Optimizing nutrient use efficiency, productivity, energetics, and economics of red cabbage following mineral fertilization and biopriming with compatible rhizosphere microbes. Scientific reports, 11(1): 1-14.
31. Suhail, F. M., and Karim, A. K.( 2017). The effect of interaction between two types of isotobacterium drainage water plate on growth and yield of wheat plant. Diyala Journal of Agricultural Sciences, 9 (1): 274-290.
32. Youssef, M. M. A., & Eissa, M. F. M. (2014). Biofertilizers and their role in management of plant parasitic nematodes. A review. Journal of Biotechnology and Pharmaceutical Research, 5(1): 1-6. | ||
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