THE POLYMORPHISM AMONG FOUR LOCAL CHICKENS IN LEI0258 MICROSATELLITE MARKER | ||
| Basrah Journal of Veterinary Research | ||
| Article 14, Volume 20, Issue 1, June 2021, Pages 150-159 PDF (1.55 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvetr.2031.175864 | ||
| Authors | ||
| Questan Ameen1; Ali Mohamed2; Sanaa Omar3; Rana Al-Obaidi4; Sehand Aref5; Ahmed Sami Shaker* 6; Hani Hermiz7 | ||
| 1Animal production department, college of agriculture, Sulaimani University, Sulaimani, Iraq. | ||
| 2Department of veterinary microbiology, College of veterinary medicine, University of Kirkuk, Kirkuk, Iraq | ||
| 3Animal production department, Technical institute-Mosul, Northern technical university, Iraq | ||
| 4College of veterinary medicine, Sulaimani University, Sulaimani, Iraq. | ||
| 5College of Science, Biology Department, Sulaimani University, Sulaimani, Iraq. | ||
| 6Animal production department, Directorate of agricultural research, Sulaimani, Iraq. | ||
| 7Animal production department, college of agriculture, Salahaddin university, Erbil, Iraq | ||
| Abstract | ||
| Major histocompatibility complex (MHC) is a cluster of genes found in vertebrate. In chicken it’s located in chromosome 16, exactly linked with LEI0258 microsatellite marker (Accession no. KF534927.1). Current study was done to sequence the LEI0258 microsatellite marker in four genetic groups of Kurdish local chicken. Which was namely Black, Black brown neck, White shank feather, and White non-feathering shank. Fresh blood samples were withdrawn from wing vein and genomic DNA was isolated from each to determine the sequences. The result showed that the sequences of the four genetic groups differ from each other. Two clusters were found among the genetic groups the first content Black and black brown neck, and the second cluster content White shank feather and the white non-feathering shank. | ||
| Keywords | ||
| Key words: Immune; MHC; chicken | ||
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