The Impacts of Calcium Ions Substitution in Hydroxyapatite with Neodymium and Zinc on Biological Properties and Osteosarcoma Cells

AL-Shahrabalee, Suha Q.; Jaber, Hussein A.

doi:10.30684/etj.2022.133915.1217

	The Impacts of Calcium Ions Substitution in Hydroxyapatite with Neodymium and Zinc on Biological Properties and Osteosarcoma Cells
Engineering and Technology Journal
Article 5, Volume 40, Issue 12, December 2022, Pages 1650-1658 PDF (678.22 K)
Document Type: Research Paper
DOI: 10.30684/etj.2022.133915.1217
Authors
Suha Q. AL-Shahrabalee^* ¹; Hussein A. Jaber²
¹Materials Engineering Department/ University of Technology- Baghdad- Iraq
²Materials Engineering Department/ University of technology- Baghdad - Iraq
Abstract
Hydroxyapatite (HA) is one of the important biomaterials in the medical field, especially in bone treatment, because of its biological properties close to human bone. A simple co-precipitation technique was used to integrate neodymium and zinc into HA by adding neodymium nitrate and zinc nitrate as a source of substituted elements during synthesis through the wet precipitation method with controlled temperature and pH. Finally, substituted HA was sintered at 800°C after completing the biomaterial preparation. The resulting Nd-Zn/HA was globe-like with nanoparticle size. The Ca+Nd+Zn/P ratio was equal to 1.63, which is relatively close to the molar ratio of bone. Also, the ability of Nd-Zn/HA to cause apoptosis in osteosarcoma cells was discovered. The anti-tumor actions are amplified when increasing the concentration of substituted HA. Therefore, Nd-Zn/HA is a potentially effective biomaterial in osteosarcoma treatment. Meanwhile, it has antibacterial and fungicidal properties against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Escherichia coli, and Candida albicans—one of the important properties required in biomaterials to protect the part that is being treated after the biomaterial is implanted inside the body. The inhibition zone of Nd-Zn/HA ranged between (20-31)mm, much higher than gentamicin and nystatin.
Highlights
The wet precipitation method can use to prepare valuable substituted hydroxyapatite. Substituted hydroxyapatite with rare earth elements (Neodymium) and Zinc have antibacterial and fungicide activity. Elements used in substitution can be led to a grateful change in the anticancer effect of Nd-Zn/hydroxyapatite. Substitute part of calcium ion with other ions can generate safe biomaterial on the human body.
Keywords
Biomaterials; Hydroxyapatite; Neodymium; Zinc; Wet precipitation method

References
[1] A. Bigi and E. Boanini, “Functionalized biomimetic calcium phosphates for bone tissue repair,” Journal of Applied Biomaterials and Functional Materials., 15 (2017) e313–e325.doi: 10.5301/jabfm.5000367. [2] R. Afif and M. Anaee, “Properties of Functionally Graded Coating of Al₂O₃ /ZrO₂ /HAP on SS 316L,” Int. J. Sci. Eng. Res., 6 (2015) 953–957. [Online]. Available: http://www.ijser.org. [3] R. A. Anaee, “Behavior of Ti/HA in Saliva at Different Temperatures as Restorative Materials,” J. Bio- Tribo-Corrosion., 2 (2016) 1–9.doi: 10.1007/s40735-016-0036-1. [4] A. Mehatlaf, A. Atiyah, and S. Farid, “An Experimental Study of Porous Hydroxyapatite Scaffold Bioactivity in Biomedical Applications,” Eng. Technol. J., 39 (2021) 977–985.doi: 10.30684/etj.v39i6.2059. [5] Y. Wang et al., “Dual functional selenium-substituted hydroxyapatite,” Interface Focus., 2 (2012) 378–386.doi: 10.1098/rsfs.2012.0002. [6] T. Kokubo, H. M. Kim, and M. Kawashita, “Novel bioactive materials with different mechanical properties,” Biomaterials., 24 (2003) 2161–2175.doi: 10.1016/S0142-9612(03)00044-9. [7] F. Witte et al., “Biodegradable magnesium-hydroxyapatite metal matrix composites,” Biomaterials., 28 (2007) 2163–2174.doi: 10.1016/j.biomaterials.2006.12.027. [8] A. M. Pietak, J. W. Reid, M. J. Stott, and M. Sayer, “Silicon substitution in the calcium phosphate bioceramics,” Biomaterials., 28 (2007) 4023–4032.doi: 10.1016/j.biomaterials.2007.05.003. [9] M. Safarzadeh et al., “Effect of multi-ions doping on the properties of carbonated hydroxyapatite bioceramic,” Ceram. Int., 45 (2019) 3473–3477.doi: 10.1016/j.ceramint.2018.11.003. [10] D. Arcos and M. Vallet-Regí, “Substituted hydroxyapatite coatings of bone implants,” Journal of Materials Chemistry B., 8 (2020) 1781–1800.doi: 10.1039/c9tb02710f. [11] T. Tite et al., “Cationic substitutions in hydroxyapatite: Current status of the derived biofunctional effects and their in vitro interrogation methods,” Materials (Basel)., 11 (2018) 1–62.doi: 10.3390/ma11112081. [12] H. Kabir, K. Munir, C. Wen, and Y. Li, “Recent research and progress of biodegradable zinc alloys and composites for biomedical applications: Biomechanical and biocorrosion perspectives,” Bioactive Materials., 6 (2021) 836–879.doi: 10.1016/j.bioactmat.2020.09.013. [13] V. Chopra et al., “Synthesis and Evaluation of a Zinc Eluting rGO/Hydroxyapatite Nanocomposite Optimized for Bone Augmentation,” ACS Biomater. Sci. Eng., 6 (2020) 6710–6725.doi: 10.1021/acsbiomaterials.0c00370. [14] G. S. Kumar, E. K. Girija, M. Venkatesh, G. Karunakaran, E. Kolesnikov, and D. Kuznetsov, “One step method to synthesize flower-like hydroxyapatite architecture using mussel shell bio-waste as a calcium source,” Ceram. Int., 43 (2017) 3457–3461.doi: 10.1016/j.ceramint.2016.11.163. [15] L. Sheikh, S. Sinha, Y. N. Singhababu, V. Verma, S. Tripathy, and S. Nayar, “Traversing the profile of biomimetically nanoengineered iron substituted hydroxyapatite: Synthesis, characterization, property evaluation, and drug release modeling,” RSC Adv., 8 (2018) 19389–19401.doi: 10.1039/c8ra01539b. [16] P. Andrea et al., “Comparative study between natural and synthetic Hydroxyapatite : structural , morphological and bioactivity properties,” Matéria (Rio de Janeiro)., 23 (2018).doi: 10.1590/S1517-707620180004.0551 [17] S. Meejoo, W. Maneeprakorn, and P. Winotai, “Phase and thermal stability of nanocrystalline hydroxyapatite prepared via microwave heating,” Thermochim. Acta., 447 (2006) 115–120.doi: 10.1016/j.tca.2006.04.013. [18] F. Bollino, E. Armenia, and E. Tranquillo, “Zirconia/hydroxyapatite composites synthesized via sol-gel: Influence of hydroxyapatite content and heating on their biological properties,” Materials (Basel)., 10 (2017) 757.doi: 10.3390/ma10070757. [19] O. Kaygili et al., “Structural and Dielectrical Properties of Ag- and Ba-Substituted Hydroxyapatites,” J. Inorg. Organomet. Polym. Mater., 24 (2014) 1001–1008.doi: 10.1007/s10904-014-0074-4. [20] M. Andrean et al., “Synthesis of hydroxyapatite by hydrothermal and microwave irradiation methods from biogenic calcium source varying pH and synthesis time,” Boletín la Soc. Española Cerámica y Vidr., 61 (2020) 35–41.doi: 10.1016/j.bsecv.2020.06.003. [21] S. Ferraris et al., “Acta Biomaterialia Bioactive materials : In vitro investigation of different mechanisms of hydroxyapatite precipitation,” Acta Biomater., 102 (2020) 468–480.doi: 10.1016/j.actbio.2019.11.024. [22] A. Destainville, E. Champion, D. Bernache-Assollant, and E. Laborde, “Synthesis, characterization and thermal behavior of apatitic tricalcium phosphate,” Mater. Chem. Phys., 80 (2003) 269–277.doi: 10.1016/S0254-0584(02)00466-2. [23] S. V. Dorozhkin, “Amorphous Calcium Orthophosphates: Nature, Chemistry and Biomedical Applications,” Int. J. Mater. Chem., 2 (2012) 19–46.doi: 10.5923/j.ijmc.20120201.04. [24] K. A. Prosolov, V. V. Lastovka, O. A. Belyavskaya, D. V. Lychagin, J. Schmidt, and Y. P. Sharkeev, “Tailoring the surface morphology and the crystallinity state of cu-and zn-substituted hydroxyapatites on Ti and Mg-based alloys,” Materials (Basel)., 13 (2020) 1–20.doi: 10.3390/ma13194449. [25] J. Zhao, Y. Liu, W. Bin Sun, and H. Zhang, “Amorphous calcium phosphate and its application in dentistry,” Chem. Cent. J., 5 (2011) 40.doi: 10.1186/1752-153X-5-40. [26] E. D. Eanes, “Amorphous Calcium Phosphate: Thermodynamic and Kinetic Considerations,” in Calcium Phosphates in Biological and Industrial Systems, Springer, Boston, MA., (1998) 21–39.doi: 10.1007/978-1-4615-5517-9_2. [27] V. Rodríguez-Lugo et al., “Wet chemical synthesis of nanocrystalline hydroxyapatite flakes: Effect of pH and sintering temperature on structural and morphological properties,” R. Soc. Open Sci., 5 (2018) 180962.doi: 10.1098/rsos.180962. [28] Harun, W. S. W., R. I. M. Asri, A. B. Sulong, S. A. C. Ghani, and Z. Ghazalli. “Hydroxyapatite–Advances in Composite Nanomaterials.” Biomedical Applications and Its Technological Facets. In Tech., 2018.doi: 10.5772/intechopen.68820. [29] D. E. Talburt and G. T. Johnson, “Some Effects of Rare Earth Elements and Yttrium on Microbial Growth,” Mycologia., 59 (1967) 492–503.doi: 10.2307/3756768. [30] D. Predoi et al., “Textural, structural and biological evaluation of hydroxyapatite doped with zinc at low concentrations,” Materials (Basel)., 10 (2017) 229.doi: 10.3390/ma10030229. [31] K. P. Tank, K. S. Chudasama, V. S. Thaker, and M. J. Joshi, “Pure and zinc doped nano-hydroxyapatite: Synthesis, characterization, antimicrobial and hemolytic studies,” J. Cryst. Growth., 401 (2014) 474-479.doi: 10.1016/j.jcrysgro.2014.01.062. [32] A. Anwar, S. Akbar, A. Sadiqa, and M. Kazmi, “Novel continuous flow synthesis, characterization and antibacterial studies of nanoscale zinc substituted hydroxyapatite bioceramics,” Inorganica Chim. Acta., 453 (2016) 16–22. doi: 10.1016/j.ica.2016.07.041. [33] S. Balu, M. V. Sundaradoss, S. Andra, and J. Jeevanandam, “Facile biogenic fabrication of hydroxyapatite nanorods using cuttlefish bone and their bactericidal and biocompatibility study,” Beilstein J. Nanotechnol., 11 (2020) 285–295.doi: 10.3762/bjnano.11.21. [34] Y. Li, C. P. Ooi, P. Cheang Hong Ning, and K. Aik Khor, “Synthesis and characterization of neodymium(III) and gadolinium(III)-substituted hydroxyapatite as biomaterials,” Int. J. Appl. Ceram. Technol., 6 (2009) 501–512.doi: 10.1111/j.1744-7402.2008.02293.x. [35] S. P. Victor, W. Paul, V. M. Vineeth, R. Komeri, M. Jayabalan, and C. P. Sharma, “Neodymium doped hydroxyapatite theranostic nanoplatforms for colon specific drug delivery applications,” Colloids Surfaces B Biointerfaces., 145 (2016) 539–547.doi: 10.1016/j.colsurfb.2016.05.067. [36] I. Kostova and M. Traykova, “Cerium ( III ) and Neodymium ( III ) Complexes as Scavengers of X / XO- Derived Superoxide Radical,” Medicinal Chemistry., 2 (.2006) 463–470. [37] I. R. De Lima et al., “Understanding the impact of divalent cation substitution on hydroxyapatite: An in vitro multiparametric study on biocompatibility,” J. Biomed. Mater. Res. - Part A, 98 (2011) 351–358.doi: 10.1002/jbm.a.33126. [38] H. Shi, Z. Zhou, W. Li, Y. Fan, Z. Li, and J. Wei, “Hydroxyapatite based materials for bone tissue engineering: A brief and comprehensive introduction,” Crystals., 11 (2021) 1–18.doi: 10.3390/cryst11020149.
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