In vivo study of Impact Transplantation Hematopoietic Progenitor Cells on Induced Cutaneous Wound Healing in Rabbits Model

Zedan, Zahraa K.; Hassan, Salah A.

doi:10.33899/ijvs.2021.130949.1899

	In vivo study of Impact Transplantation Hematopoietic Progenitor Cells on Induced Cutaneous Wound Healing in Rabbits Model
Iraqi Journal of Veterinary Sciences
Article 5, Volume 36, Issue 3, July 2022, Pages 579-589 PDF (934.78 K)
Document Type: Research Paper
DOI: 10.33899/ijvs.2021.130949.1899
Authors
Zahraa K. Zedan¹; Salah A. Hassan^* ²
¹Department of molecular and medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq
²Departments of Surgery and Obstetrics, College of Veterinary Medicine, Al- Qasim Green University, Babylon, Iraq
Abstract
The goal of this study was to assess the effects of the Hematopoietic Progenitor Cells (HSCs) on full-thickness cutaneous wounds healing in rabbits. Twenty clinically healthy adult New Zealand White rabbits were used in this study; under aseptic technique and general anesthesia full-thickness excisional cutaneous wound; 20 mm x 20 mm were made on the back. The rabbits were randomly partitioned into two. In first group (control group), the wounds were treated with 3ml of saline solution. In second group (Hematopoietic Progenitor Cells- group); wounds were treated with 5x106 (Hematopoietic Progenitor Cells). The result of molecular evaluation along the interval of following-up recorded a significant differences (P<0.05) of the level Fibroblasts Growth Factor (FGF) and Vascular Endothelial Growth Factor (VEGF) recorded at 7, 14 and 35 day post-surgical operation in (Hematopoietic Progenitor Cells- group) compare than control group. These outcomes were parallel with the finding of the clinical and histopathological assessment showed that Hematopoietic Progenitor Cells- group reduces inflammation and promotes epithelialization during 3th weeks with increased vasculature than those in untreated wounds. This study confirms that local implantation of Hematopoietic Progenitor Cells leads to enhance and develop of cutaneous wound healing in rabbit model.
Highlights
Article Highlight Hematopoietic Progenitor Cells (HSCs) were created in a laboratory setting (issue Culture Unite, Department of Biotechnology, Al-Nahrain University). Normal wound healing is a dynamic and complex multiple phase process involving coordinated interactions between growth factors, cytokines, chemokines, and various cells. Any failure in these phases may lead wounds to become chronic and have abnormal scar formation. Stem cell application in wound care is to target improved quality of wound healing by acceleration in healing, prevention of wound contracture and scar formation and earlier wound closure. The most stem cells used in skin regeneration and wound healing are adult stem cells owing to containing significant proliferative capacity, long-term self-renewal potential, and having the ability to differentiate into other lineages.. Transplantation of Hematopoietic Progenitor Cells (HSCs) on full-thickness cutaneous wounds healing in rabbits significantly accelerated wound healing and remarkably decreased the quantity of infiltrated inflammatory cells and increased the level of VEGF and b-FGF gene in wound by promoted wound angiogenesis.
Keywords
Cutaneous wounds,,; ,،,؛HSCs,,; ,،,؛rabbit,,; ,،,؛wounds healing

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