An Evaluation of Cytotoxicity of Iron Oxide Nanoparticles with Hydrogen Peroxide Endodontic Irrigant | ||
| Al-Rafidain Dental Journal | ||
| Article 1, Volume 23, Issue 1, March 2023, Pages 1-8 PDF (464.12 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/rden.2021.129103.1069 | ||
| Authors | ||
| Saraa Al-Mallah* 1; Ali Al-Naimi2 | ||
| 1Conservative department. College of Dentistry, University of Mosul, Iraq | ||
| 2Conservative Department, College of Dentistry, University of Mosul, Iraq | ||
| Abstract | ||
| Aims: The aim of this research was to determine the cytotoxic effect of different concentrations of iron oxide nanoparticles (IONPs) in combination with hydrogen peroxide ( H2O2) in vitro as a potential endodontic irrigating solution. Materials and Methods: Cytotoxicity of IONPs at concentrations (8, 4, 2, 1, 0.5, and 0.25 mg/ml) with 3% H2O2 were evaluated on cultured Human Dermal Fibroblast (HDFn) cell line. Cytotoxicity was assessed after 10 minutes of exposure using the Mosmann’s Tetrazolium Toxicity (MTT) assay. Results: Tukey’s test indicated that no significant difference in cell viability was found at lower concentrations (0.2, 0.5, 1, and 2 mg/ml). While at a concentration of 4 and 8 mg/ml, the cell viability was not significantly different from each other whereas, significantly different from previous concentrations. Conclusion: From the gained data, it can be deduced that IONPs+H2O2 had almost no cytotoxic effect on HDFn cell viability at concentrations 0.2, 0.5, 1, and 2 mg/ml, while a moderate decrease of survival has been observed at concentrations 4 and 8 mg/ml. | ||
| Keywords | ||
| Cytotoxicity; human dermal fibroblast cells; hydrogen peroxide; Iron oxide; Irrigating solutions | ||
|
Statistics Article View: 469 PDF Download: 223 |
||