THE ASSOCIATION OF THE POLYMORPHISM AND GENE EXPRESSION OF HEAT SHOCK PROTEIN HSP70 GENE IN WINTER AND SUMMER IN THE SEMEN OF HOLSTEIN BULLS BORN IN IRAQ | ||
Basrah Journal of Veterinary Research | ||
Article 23, Volume 17, 3 (Proceeding of 6th International Scientific Conference, College of Veterinary Medicine University of Basrah, Iraq), November 2018, Pages 280-289 PDF (215.86 K) | ||
Document Type: Research Paper | ||
DOI: 10.23975/bjvetr.2018.172991 | ||
Authors | ||
Hassan Nima Habib* 1; Bassam Yasein Khudaier2; Amad Falah Hassan1; Wessam M M Saleh2 | ||
1College of Agriculture, University of Basrah, Basrah, Iraq | ||
2College of Veterinary, Medicine University of Basrah, Basra, Iraq | ||
Abstract | ||
The HSP70 protein considered as a molecular markers to resist various stress conditions, especially high temperatures. Therefore, the high level of gene expression of hsp70 gene is one of the ways to resist stress conditions, which can vary depending on the polymorphisms. The study aimed to determine the extent of the correlation between gene expression and polymorphisms to hsp70 gene. The study was conducted in the months: November , December , 2015, January 2016, as the winter season and the months April , May and June, 2016, as the summer season. Twenty nine Holstein bulls born in Iraq of known fertility, 2.5 – 3.5 years old, back to Artificial Insemination Center of Abou-Ghareeb Baghdad, Iraq, the General Company for Livestock Services, Semen collected using artificial vagina method, mRNA extraction, Synthesis of mRNA to cDNA and Real-Time Quantitative RT–qPCR were done. The results showed significant differences in the level of gene expression between polymorphisms to the hsp70 gene. In conclusion there is a positive correlation between the gene expression and polymorphisms of hsp70 gene in Holstein bulls born in Iraq . | ||
Keywords | ||
HSP70 protein; gene expression; polymorphisms | ||
Full Text | ||
Basrah Journal of Veterinary Research,Vol.17, No.3,2018 | ||
References | ||
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