DETECTION OF INTRACELLULAR ADHESION GENE (icaA and icaD)AND BIOFILM FORMATION STAPHYLOCOCCUS AUREUS ISOLATES FROM MASTITIS MILK OF SHEEP AND GOAT | ||
| Basrah Journal of Veterinary Research | ||
| Article 10, Volume 18, Issue 2, December 2019, Pages 306-327 PDF (557.51 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvetr.2022.172828 | ||
| Authors | ||
| Mohammed Hasan Khudor* ; Hasan I. Idbeis | ||
| Department of Microbiology, College of Veterinary Medicine ,University of Basrah, Basrah,Iraq | ||
| Abstract | ||
| In the present study, a total of 150 mastitis milk samples were collected from sheep and goat (75 for each one) and were analyzed for the presence of S.aureus.. The obtained results indicate that this bacterium observed in 20% of these samples (21.33% from sheep and 18.66% from goat) .The study of antibiotic susceptibility test to 9 different antibiotics showed that S. aureus was 100% resistant to penicillin and 100% sensitive to vancomycin, gentamycin , clarithromycin and chloramphenicol . whereas for cefoxitin (alternative to methicillin) resistance was 47%.There were a variable sensitivity percentage for the rest of antibiotics: Tetracycline (70%), Ciprofloxacin (80%) , Clindamycin (83%). The biofilm-forming ability of S. aureus was evaluated via microtiter plates and the result revealed that, all the studied isolates were either moderate biofilm producer or weak biofilm producer while the non-biofilm producer and strong biofilm producer were not detected among the tested isolate.The relationship between biofilm formation and resistance to methicillin showed there as no significant differences (P>0.05) in the percentage of weak and moderate biofilm producers between MRSA and MSSA isolates. PCR analysis was applied to DNA extracted from S.aureus isolates from milk samples .The results of PCR assay revealed that all S.aureus isolates gave positive results for both icaA and icaD genes (100%) with Product size 151 and 211 bp, respectively. Results of this study indicate that biofilm producing S.aureus have a major role player on the occurrence of mastitis .In addition, there was high prevalence of MRSA isolates (47%) in mastitic milk at the study area | ||
| Keywords | ||
| Staphylococcus aureus; Mastitis; biofilm | ||
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