PATHOLOGICAL AND MOLECULAR EVALUATION OF CANINE PARVOVIRUS IN SOUTH OF IRAQ | ||
| Basrah Journal of Veterinary Research | ||
| Article 4, Volume 18, Issue 2, December 2019, Pages 185-194 PDF (530.3 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvetr.2022.172809 | ||
| Authors | ||
| Mansoor Al-Khaled1; ِِAsmaa Al-Jawasim* 2; Marid Nasser Auda3; Jihad Abdulameer Ahmed* 3 | ||
| 1Department of Microbiology, Veterinary Medicine College, Al-Qadisiyah University, Al-Diwaniyah, Iraq | ||
| 2Department of Microbiology, Veterinary Medicine College, Al-Qadisiyah University, Al- Diwaniyah, Iraq. | ||
| 3Department of Pathology and poultry diseases., College of Veterinary Medicine,University of Basrah,Basrah,Iraq | ||
| Abstract | ||
| This study is the first in southern Iraq, which shows the diagnosis of Parvovirus disease in young puppies, where the samples were collected from small dogs, and this was done from April 2018 to April 2019 it was conducted on 50 dogs with different breeds ranging between (2-18) months aged of both sexes. fecal samples were collected from infected dogs from veterinary clinics in (Basrah, Dhi Qar, Maysan and AL-Muthanna provinces) .After the autopsy of the dead carcass which died soon during the period (24-72) hours, bleeding in the intestines and congestion was observed as well as the water content and adhesions, hypertrophy of the heart and necrosis in areas of pale color of different size focci are surrounded by hyperemic zone. The microscopical result of enteric CPV lesion showed sever infiltration of inflammatory cells in the mucosal layer of intestine ,hypertrophy of goblet cells in the villi and necrotizing area in the tip of villi as well to hyperplasia of goblet cells also edematous fluid in mucosal layer. cardiac CPV lesion showed infiltration of inflammatory cells in myocardial cell fibers ,also there was an area of vacculation of some myocardial cells in addition to edematous fluid in the myocardial interstitiaum .significant increases of biochemical cardiac markers(cTi-1,AST,LDH and CPK) showed cardiac troponin I (cTnI), aspartate transaminase AST, creatinine phosphatkinase (CPK), and lactate dehydrogenase LDH which showed (0.024 ± 0.003),( 50.5±4.49),( 327.9 ± 55.01) and (467.9 ±49.1) respectively when it is compared to healthy group which showed cardiac troponin I (cTnI), aspartate transaminase AST, creatinine phosphatkinase (CPK), and lactate dehydrogenase LDH were (0.004± 0.0003),( 24.2±1.74),( 90± 13.58) and (289 ±15.5), the Parvovirus contains 400 bp of matrimonial bases after being diagnosed using the polymerase chain reaction PCR and this technical method widely applied to provide rapid diagnosis The current study conducts that the CPV-2 is endemic in south of Iraq ,the disease shows very important clinical ,pathological and biochemical feature that lead to increase morbidity and mortality rate. | ||
| Keywords | ||
| pathological; molecular; canine parvovirus | ||
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