Plasmid –mediated antimicrobial resistance bla CMY-2 gene and qnr (qnr A,qnr B and qnr S) genes in Salmonella isolated from human, animal and milk | ||
| Basrah Journal of Veterinary Research | ||
| Article 3, Volume 20, Issue 2, December 2021, Pages 31-43 PDF (792.88 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bvetr.2021.170496 | ||
| Authors | ||
| Mohammed Khudor* ; Marwan Mohammed | ||
| Department of microbiology, College of Veterinary Medicine, University of Basrah, Iraq | ||
| Abstract | ||
| This study was conducted to detect the blaCMY-2 gene and qnr genes (qnrA, qnrB and qnrS) in Salmonella isolates from 278 different samples (50 direct milk samples, 50 indirect milk samples, 50 feces samples, 50 teat swab samples, 28 manual milk swabs and 50 stool samples) in Basrah province. The results showed that the percentage of Salmonella isolates in the samples was 6.1% by using API system and by PCR technique for identification. The highest resistance to Salmonella isolates were found against chloramphenicol and rifampin (100%). While all isolates were sensitive to ciprofloxacin . The use of plasmid treatment (Plasmid curing) by temperature method showed that 41.1% of total Salmonella isolates were associated with antimicrobial resistance of the plasmid. Plasmid analysis by molecular detection revealed that 11 isolates (64.7%) was positivity for blaCMY-2 while the qnr quinolone gene (A, B and S) was not detected in the isolates | ||
| Keywords | ||
| Key words: Plasmid curing; antimicrobial resistance genes; Salmonella; human; animals; Milk | ||
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