Thanoon, H., Alazzo, N. (2020). The Role of Calretinin Immunohistochemistry in Detection of Ganglion Cell in Hirschsprung Disease. Alustath, 20(4), 368-372. doi: 10.52573/ipmj.2020.170483
Hawazin Hazim Thanoon; Nadwa Subhi Alazzo. "The Role of Calretinin Immunohistochemistry in Detection of Ganglion Cell in Hirschsprung Disease". Alustath, 20, 4, 2020, 368-372. doi: 10.52573/ipmj.2020.170483
Thanoon, H., Alazzo, N. (2020). 'The Role of Calretinin Immunohistochemistry in Detection of Ganglion Cell in Hirschsprung Disease', Alustath, 20(4), pp. 368-372. doi: 10.52573/ipmj.2020.170483
Thanoon, H., Alazzo, N. The Role of Calretinin Immunohistochemistry in Detection of Ganglion Cell in Hirschsprung Disease. Alustath, 2020; 20(4): 368-372. doi: 10.52573/ipmj.2020.170483

The Role of Calretinin Immunohistochemistry in Detection of Ganglion Cell in Hirschsprung Disease

Iraqi Postgraduate Medical Journal
Article 11, Volume 20, Issue 4, October 2021, Pages 368-372    PDF (161.38 K)
Document Type: Research Paper
DOI: 10.52573/ipmj.2020.170483
Authors
Hawazin Hazim Thanoon1; Nadwa Subhi Alazzo2
1Histopathology/ Mosul Center of Pathology, Nineveh, Iraq
2Department of Pathology /College of Medicine/ University of Mosul, Nineveh,Iraq
Abstract
ABSTRACT:
BACKGROUND:
Hirschsprung’s disease is a developmental abnormality of enteric nervous system characterized by lack of ganglion cell and neural hypertrophy in the Meissner and Auerbach’s plexuses within variable length of the colon, the Calretinin Immunohistochemistry is one of the newer methods overcome difficulties in diagnosis of Hirschsprung’s disease especially immature ganglia in neonates in submucosal plexus.
AIM OF STUDY:
This study was performed to evaluate the diagnostic value of Calretinin immunohistochemistry in                 the Hirschsprung’s Disease diagnosis.
MATERIALS AND METHOD:
A retrospective and prospective case series study at the period from (Jan2018-Jan2020) included a total 50 formalin-fixed paraffin-embedded blocks of distal colonic and rectal incisional biopsies from infant and young children patient who suspected with Hirschsprung’s disease, The study was undertaken in the department of pathology, collage of medicine/ University of Mosul, a clinicopathological data including (age, gender, site, type of surgical procedure) were obtained from patients' files This study was performed by using the primary antibody of Calretinin.
RESULTS:
The study done on 50 cases their age from 1 day to 14 years. The universal sensitivity in this study to diagnose presence or loss of enteric ganglia by calretinin immunostaining was 90% and the specificity is 100% with positive and negative predictive value of 100% and 97 % respectively.
CONCLUSION:
Calretinin marker considered as a reliable, valuable marker in Hirschsprung disease diagnosis and carry high sensitivity and specificity.
 
 
Keywords
Hirschsprung disease; Calretinin
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