Spectrophotometric Determination of Mebendazole using Diazotization Reaction and Coupling with m-Aminophenol Reagent | ||
| Rafidain Journal of Science | ||
| Article 1, Volume 30, Issue 4, December 2021, Pages 1-10 PDF (482.9 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/rjs.2021.169972 | ||
| Authors | ||
| Rowa Falah Mohammed* 1; Farha Kh. Omar2 | ||
| 1Department of Chemistry, College of Education for Girls, Mosul University, Mosul, Iraq. | ||
| 2Department of Chemistry/ College of Education for Girls/ University of Mosul | ||
| Abstract | ||
| A simple and sensitive spectrophotometric method has been developed for the determination of mebendazole (MBZ) in it's pure and tablet form. The method is based on alkaline hydrolysis of MBZ with sodium hydroxide to give primary amine product which reacts with sodium nitrite in an acidic medium (hydrochloric acid) to yield diazotized mebendazole (D-MBZ) which is coupling with m-aminophenol reagent (mAP) to form an azo dye. The absorbance of the azo dye for this suggested method (yellow dye) has been measured at 360 nm. Beer's law for this proposed method is in the range of (0.5-20 µg.ml-1), the molar absorptivity value is 1.63×104 L.mol-1.cm-1 and the Sandell's value index is calculated and equal to 0.018 μg.cm- 2. Also, the limit of detection and the limit of quantification are calculated and equal to 0.1718 μg.ml-1 and 0.5726µg.ml-1 respectively. The ratio of the formed azo dye [D-MBZ: mAP] is [2: 1]. | ||
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