Extracellular Enzymes of Endophytic Fungi Hosted Salt Marsh Plants in the South Eastern, Algeria | ||
| Al-Qadisiyah Journal For Agriculture Sciences | ||
| Article 5, Volume 11, Issue 2, December 2021, Pages 26-31 PDF (412.95 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33794/qjas.2021.131865.1010 | ||
| Authors | ||
| Wassima Lakhdari* 1; Randa Mlik1; Hamida Hammi2; Ibtissem Benyahia3; Nour Elhouda Mekhadmi4; Berrekbia Mohammed5; Sofiane Gheriani5; Abderrahmene Dehliz5 | ||
| 1National Institute of Agronomic Research of Algeria, INRAA | ||
| 2National Institute of Agronomic Research of Algeria, INRAa | ||
| 3Department of Chemistry, Faculty of Mathematics and Material Sciences, Laboratory of Biogeochemistry and Desert environments, University of Kasdi Merbah, Ouargla | ||
| 4Biology Department, University of El-Oued, Algeria | ||
| 5National Institute of Agronomic Research of Algeria | ||
| Abstract | ||
| Endophyte microorganisms have great biotechnological interest, with features applicable to different areas and are potentially useful in agriculture. In the current study, the most dominant and representative endophytic fungal species of seven halophytic plants prevalent in the southeastern Algeria, Touggourt, were screened for their ability to produce four extracellular enzymes namely: cellulase, amylase, laccase, and lipase. Zygophyllum album came first by hosting diverse endophytic species among all the tested plants with eight species followed by Tamarix boveana and Limoniastrum guyonianum (46.66%) as well as Phragmites communis with 40% and Haloxylon articulatum and Aeluropus littoralis with 33.33%. Unlike J. effusus, it was the less diverse plant where only three species (20%) were identified. In term of enzymatic activities of the selected fungi, the two species of Chaetomium presented cellulase, amylase and lipase activity. Unlike, Trichoderma harzianum involved in lipase, Ulocladium sp. in cellulase, Bipolaris sp. and Botryostimphylium sp. in Amylase. Unlike, the two species of Chaetomium involved in cellulase, amylase and lipase. On the other hand, no enzyme activity was recorded in the colonies of Fusarium sp. | ||
| Keywords | ||
| Halophyte,,; ,،,؛endophytic fungi,,; ,،,؛enzyme activity,,; ,،,؛cellulase,,; ,،,؛Sahara,,; ,،,؛Algeria | ||
| References | ||
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[1] Petrini O. (1991). Fungal endophytes of tree leaves. In: Andrews JH, Hirano SS, editors. Microbial Ecology of leaves. New York: Springer. 179-197.
[2] Azevedo J. L., Maccheroni J.r.W., Pereira J. O. & Araújo W. L. (2000). Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electron J Biotechnol., 3, 1, 15-6.
[3] Sudha V., Govindaraj R., Baskar K., Al-Dhabi N. A., Duraipandiyan V. (2016). Biological properties of endophytic fungi. Braz Arch Biol Technol. 59: e16150436.
[4] Tan R. X., Zou W.X. (2001). Endophytes: a rich source of functional metabolites. Nat Prod Rep. 18: 448-59.
[5] Strobel G. A. (2003). Endophytes as sources of bioactive products. Microbes and Infection. 5: 535-44.
[6] Strobel G., Daisy B., Castillo U., Harper J. (2004). Natural products from endophytic microorganisms. J Nat Prod. 67 (2): 257-68.
[7] Maria G. L., Sridhar K. R., Raviraja N. S. (2005). Antimicrobial and enzyme activity of mangrove endophytic fungi of southwest coast of India. Journal of Agricultural Technology.
[8] Rajamanikyam M., Vadlapudi V., Upadhyayula S. M. (2017). Endophytic fungi as novel resources of natural therapeutics. Braz Arch Biol Technol. 60: 1-7.
[9] Dutta D., Puzari K. C., Gogoi R., Dutta P. (2014). Endophytes: exploitation as a tool in plant protection. Braz Arch Biol Technol. 57, 5, 621-29.
[10] Kusari S., Singh S., Jayabaskaran C. (2014). Rethinking production of Taxol® (paclitaxel) using endophyte biotechnology. Trends Biotechnol., 32, 6, 304-11.
[11] Nisa H., Kamili A. N., Nawchoo I. A., Shafi S., Shameem N., Bandh A. S. (2015). Fungal endophytes as prolific source of phytochemicals and other bioactive natural products: a review. Microb Pathog., 82, 50-9.
[12] Qadri M., Nalli Y., Jain S. K., Chaubey A., Ali A., Strobel G. A., et al. (2017). An insight into the secondary metabolism of Muscodor yucatanensis: small-molecule epigenetic modifiers induce expression of secondary metabolism-related genes and production of new metabolites in the endophyte. Microb Ecol. 73 (4): 954-65.
[13] Ding C. H., Wang Q. B., Guo S., Wang Z. Y. (2018). The improvement of bioactive secondary metabolites accumulation in Rumex gmelini Turcz through co-culture with endophytic fungi. Braz J Microbiol., 49, 2, 362-69.
[14] Anbu P., Gopinath S. C. B., Cihan A. C. & Chaulagain B. P. (2013). Microbial enzymes and their applications in industries and medicine. BioMed.
[15] Sunitha V. H., Nirmala Devi D., Srinivas C. (2013). Extracellular Enzymatic Activity of Endophytic Fungal Strains Isolated from Medicinal Plants. World Journal of Agricultural Sciences. 9 (1): 01-09.
[16] Desire M. H., Bernard F., Forsah M. R., Assang C. T., et al. (2014). Enzymes and qualitative phytochemical screening of endophytic fungi isolated from Lantana camara Linn. leaves. J. Appl. Biol. Biotechnol., 2, 1-6.
[17] Abdel-Azeem A. M. (2010). The history, fungal biodiversity, conservation, and future perspectives for mycology in Egypt. IMA Fungus, 1, 2, 123–142.
[18] Lakhdari W., Dehliz A., Mlik R., Benlamoudi W., Hammi H., Fathallah R., Benyahia I., Mekhadmi N. E., Acheuk F., Ouargli D. (2020). Euphorbia guyoniana Ethanolic Extract Efficiency Against Tomato Leaf Miner in Southeastern Algeria. Research Journal of Medicinal Plants. 14: 1-7.
[19] Lakhdari W., Dehliz A., Acheuk F., Mlik R., Hammi H., Doumandji-Mitiche B., Gheriani S., Berrekbia M., Guermit K., Chergui S. (2016). Ethnobotanical study of some plants used in traditional medicine in the region of Oued Righ (Algerian Sahara). Journal of Medicinal Plants Studies, 4, 2, 204-211.
[20] Amirita A., Sindhu P., Swetha J., Vasanthi N. S. & Kannan K. P. (2012). Enumeration of endophytic fungi from medicinal plants and screening of extracellular enzymes. World Journal of Science and Technology, 2, 2, 13-19.
[21] German D. P., Weintraub M. N., Grandy A. S., Lauber C. L., Rinkes Z. L., Allison S. D. (2011). Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies. Soil Biol Biochem., 43, 1387–1397.
[22] Duncan S. M., Minasaki R., Farrell R. L., Thwaites J. M., Held B. W., Arenz B. E., Jurgens J. A., Blanchette R. A. (2008). Screening fungi isolated from historic Discovery Hut on Ross Island, Antarctica for cellulose degradation. Antarct Sci., 20, 463–470.
[23] Bradner J. R., Gillings M., Nevalainen K. H. M. (1999). Qualitative assessment of hydrolytic activities in Antarctic microfungi grown at different temperatures on solid media. World J Microb Biot., 15, 131–132.
[24] Chlebicki A. (2009). Some endophytes of Juncus trifidus from Tatra Mts. in Poland. Acta Mycol., 44, 1, 11–17.
[25] Tarroum M., Ben Romdhane W., Mohamed Ali A. A., Al-Qurainy F., Al-Doss A., Fki L., Hassairi A. (2021). Harnessing the Rhizosphere of the Halophyte Grass Aeluropus littoralis for Halophilic Plant-Growth-Promoting Fungi and Evaluation of Their Biostimulant Activities. Plants, 10, 784, 1-17.
[26] Gashgari R., Gherbawy Y., Ameen F. & Alsharari S. (2016). Molecular Characterization and Analysis of Antimicrobial Activity of Endophytic From Medicinal Plants in Saudi Arabia. Jundishapur J Microbiol., 9, 1, e26157.
[27] Bickford W. A., Bourke K. A., Clay K., Kowalski K. P., Shearin Z. R. C. (2016). Diversity of fungal endophytes in non-native Phragmites australis in the Great Lakes. Biol Invasions, 18, 2703–2716.
[28] Soares M. A., Li H. Y., Kowalski K. P., Bergen M., Torres M. S. & White J. F. (2016). Evaluation of the functional roles of fungal endophytes of Phragmites australis from high saline and low saline habitats. Biol Invasions, 18, 9, 2689–2702.
[29] El-Morsy E. M. (2000). Fungi isolated from the endorhizosphere of halophytic plants from the Red Sea Coast of Egypt. In: Aquatic Mycology across the Millennium. Fungal Diversity, 5, 43-54.
[30] Pandey P. K., Singh S., Yadav R. N. S., Singh A. K. and Chandra Kumar Singh M. (2014). Fungal endophytes: promising tools for pharmaceutical science. International Journal of Pharmaceutical Sciences Review and Research, 25, 2, 128–138.
[31] Hala M. O. R., Adel A. E., Hassaan A. E., Samed A. D. (2010). Production, optimization and partial purification of lipase from Fusarium oxysporum. Journal of Applied Sciences in Environmental Sanitation, 5, 39–53.
[32] Katoch M., Aseem S., Gurpreet S. (2014). Endophytic fungi found in association with Bacopa monnieri as potential producers of industrial enzymes and antimicrobial bioactive compounds. Brazilian Archives of Biology and Technology, 57, 714–722.
[33] Abdel-Azeem A. M. & Salem Fatma M. (2012). Biodiversity of laccase producing fungi in Egypt. Mycosphere, 3, 6, 900–920.
[34] Abdel-Azeem A. M., Gherbawy Y. A. & Sabry A. M. (2016). Enzyme profiles and genotyping of Chaetomium globosum isolates from various substrates. Plant Biosystems, 150, 3, 420-428. | ||
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