Asker, M., Rasheed, M. (2020). Analysis of Malondialdehyde and Reduced Glutathione in Spermatozoa of Infertile Men and Their Relation with Sperm Quality Parameters. Alustath, 20(3), 297-302. doi: 10.52573/ipmj.2020.169738
Mayssaa A Asker; Manal K Rasheed. "Analysis of Malondialdehyde and Reduced Glutathione in Spermatozoa of Infertile Men and Their Relation with Sperm Quality Parameters". Alustath, 20, 3, 2020, 297-302. doi: 10.52573/ipmj.2020.169738
Asker, M., Rasheed, M. (2020). 'Analysis of Malondialdehyde and Reduced Glutathione in Spermatozoa of Infertile Men and Their Relation with Sperm Quality Parameters', Alustath, 20(3), pp. 297-302. doi: 10.52573/ipmj.2020.169738
Asker, M., Rasheed, M. Analysis of Malondialdehyde and Reduced Glutathione in Spermatozoa of Infertile Men and Their Relation with Sperm Quality Parameters. Alustath, 2020; 20(3): 297-302. doi: 10.52573/ipmj.2020.169738

Analysis of Malondialdehyde and Reduced Glutathione in Spermatozoa of Infertile Men and Their Relation with Sperm Quality Parameters

Iraqi Postgraduate Medical Journal
Article 14, Volume 20, Issue 3, July 2021, Pages 297-302    PDF (200.75 K)
DOI: 10.52573/ipmj.2020.169738
Authors
Mayssaa A Asker; Manal K Rasheed
Department of Chemistry and Biochemistry / Medicine College / Baghdad University.
Abstract
BACKGROUND:
Male factor infertility is a multifactorial condition that is observed in the infertile couples. However, a proportion of infertile men have now been shown to have defective sperm functions related to oxidative stress (OS) which is a significant pathology,
AIM OF THE STUDY:
To estimate the indicators of OS by measuring malondialdehyde (MDA) and reduced glutathione (GSHr) levels in sperms of the infertile and fertile men and to evaluate the correlations of them with sperm quality parameters.
METHODS:
The levels of MDA and GSHr were measured spectrophotometrically at The Higher Institute of Infertility Diagnosis and Assisted Reproductive Technique /Al-Nahrain University, Baghdad, Iraq, between February till August 2019.
RESULT:
It was found that the mean of MDA levels was significantly higher in spermatozoa of infertile men (P ≤ 0.0001) and mean of GSHr levels was significantly lower in spermatozoa of infertile men compared to spermatozoa of fertile controls (P ≤ 0.0001). Furthermore, there were no statistical significant correlations between MDA and GSHr with sperm quality parameters.
CONCLUSION:
Evaluation of MDA and GSHr levels in spermatozoa of the infertile men provide valuable assays for estimation of OS which help in planning treatment for male infertility.
Keywords
Glutathione; male infertility; Malondialdehyde; oxidative stress; reactive oxygen species; Spermatozoa
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