Synthesis and Characterization of Chitosan- Polyvinyl alcohol Blend Modified by Genipin and Nanohydroxyapatite for Bone Tissue Engineering | ||
| Engineering and Technology Journal | ||
| Article 4, Volume 37, 11A, November 2019, Pages 470-474 PDF (681.9 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.30684/etj.37.11A.4 | ||
| Authors | ||
| Ishraq A. Kadhim1; Zuhair J. Abdul Ameer2; Assel B. Alzubaidi1 | ||
| 1Materials Engineering Department, University of Technology - Iraq | ||
| 2Engineering collage, University of Kerbela - Iraq | ||
| Abstract | ||
| Use nanohydroxyapatite into the polymeric matrix as bioactive material for bone tissue engineering has enormous therapeutic potential because beneficial properties biocompatibility, biodegradability, and consider a major inorganic constituent of the bone matrix. The blended films of Chitosan and Polyvinyl alcohol with Genipin as cross-link agent were studied with and without addition Nanohydroxyapatite. Samples were prepared by solvent casting. The resulting films blended composite were characterized by Fourier transfer infrared (FTIR) spectroscopy, degradation behavior, swelling degree and tensile strength. Degree of swelling, and weight loss of the films blended composite was decreased with an increase of genipin and nanohydroxyapatie concentrations while tensile strength was increased with an increase of genipin and nanohydroxyapaite concentrations. The results showed that the chitosan composite could be used as effective biomaterials for bone regeneration engineering with different degradation rates. | ||
| Keywords | ||
| Chitosan/Polyvinyalcohol blend; Genipin; Nanohydroxyapatite; Bone engineering; Degradation | ||
| References | ||
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[1] V.K. Malesu, D. Sahoo, P.L. Nayak, “Chitosan–Sodium Alginate Nanocomposites Blended with Cloisite 30b as a Novel Drug Delivery System for Anticancer Drug Curcumin,” International Journal of Applied Biology and Pharmaceutical Technology, Vol. 2, 2011. [2] M.M. Souza, G. Carvalho, T. Montenegro, E. Santos, P. Tenório, F. Sampaio, “Chitosan as an Oral Antimicrobial Agent,” Science against Microbial Pathogens: Communicating Current Research and Technological Advances, 2011. [3] R. Goy, D. Britto, O. Assis, “A Review of the Antimicrobial Activity of Chitosan,” Polímeros Ciência e Tecnologia, vol. 19, pp. 241-247, 2009. [4] L. Suna, J. Suna, L. Chenc, P. Niua, X. Yanga, Y. Guoa, “Preparation and Characterization of Chitosan Film iIncorporated with Thinned Young Apple Polyphenols as an Active Packaging Material,” Carbohydrate Polymers, 2017. [5] B. Manickam, R. Sreedharan, M. Emalia, ‘Genipin’ – The Natural Water Soluble Crosslinking Agent and Its Importance in the Modified Drug Delivery Systems: An Overview,” Current Drug Delivery, Vol.11, No.1, 2014. [6] J, Venkatesan, S.K. Kim, “Effect of Temperature on Isolation and Characterization of Hydroxyapatite from Tuna (Thunnus obesus) Bone,” Materials, Vol. 3, pp. 4761-4772M, 2010. [7] M.A. Seyed, K. Vijayaraghavan, “Physicochemical Characterization and Bioactivity of an Improved Chitosan Scaffold Cross-Linked With Polyvinyl Alcohol for Corneal Tissue Engineering Applications,” Annual Research & Review in Biology, Vol. 24, pp. 1-16, 2018. [8] T. Nguyen, R. Ventura, Y. Ki Min, B. Taek Lee, “ Genipin Cross-Linked Polyvinyl AlcoholGelatin Hydrogel for Bone Regeneration,” J. Biomedical Science and Engineering, Vol. 9, pp. 419-429, 2016. [9] I.H. Kalfas, M.D, F.A.C.S, “Principles of bone healing,” Neurosurg. Focus, Vol. 10, 2001. [10] J. Ai, M. Rezaei-Tavirani, M. Biazar, S. Heidari, R. Jahandideh, “Mechanical Properties of Chitosan-Starch Composite Filled Hydroxyapatite Micro-and Nanopowders,” Journal of Nanomaterials, 2011. [11] C. Peniche, Y. Solís, N. Davidenko, R. García, “Chitosan /Hydroxyapatite-Based Composites,” Biotecnología Aplicada Vol.27, No.3, 2010. [12] Z. Hu, P. Hong, M. Liao, S. Kong, N. Huang, Ch. Ou, S. Li, “ Preparation and Characterization of Chitosan—Agarose Composite Films,” Materials,Vol. 9, pp. 816-824, 2016. [13] S. Bhuvaneshwari, D. Sruthi, V. Sivasubramanian, Niranjana kalyani, J. Sugunabai, “Development and Characterization of Chitosan Film,” International Journal of Engineering Research and Applications, Vol. 1, pp. 292-299, 2011. [14] L. Bi, Z. Cao, Y. Hu, Y. Song, L. Yu, B. Yang, J. Mu, Z. Huang, Y. Han, “Effects of Different Cross-linking Conditions on the Properties of Genipin – Cross - Linked Chitosan / Collagen Scaffolds for Cartilage Tissue Engineering,” J Mater Sci: Mater Me, Vol. 22, pp. 51–62, 2011. [15] Th. Han1, N. Nwe, T. Furuike, S. Tokura, H. Tamura, “ Methods of N-Acetylated Chitosan Scaffolds and it's in Vitro Biodegradation by lysozyme,” J. Biomedical Science and Engineering, Vol. 5, pp. 15-23, 2012. [16] S. Dimida, A. Barca, N. Cancelli, V.D. Benedictis, M.G. Raucci, CH. Demitri, “Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features,” International Journal of Polymer Science, pp. 8, 2017. [17] M.E. Frohbergh, A.Katsman, G.P.Botta, Ph.Lazarovici, C.L. Schaner, U. Wegst, P.Lelkes. “Electrospun Hydroxyapatite-Containing Chitosan Nanofibers Crosslinked with Genipin for Bone Tissue Engineering,” Biomaterials, Vol. 33, pp. 9167-9168, 2012. [18] S.N. Alhosseini, F. Moztarzadeh, M. Mozafari, Sh. Asgari, M. Dodel, A. Samadikuchaksaraei, S. Kargozar, N. Jalali1 “Synthesis and Characterization of Electrospun Polyvinyl Alcohol Nanofibrous Scaffolds Modified by Blending with Chitosan for Neural Tissue Engineering,” International Journal of Nanomedicine, Vol 7, pp. 25–34, 2012. [19] N. Siddiqui, K. Pramanik, E. Jabbari, “Osteogenic Differentiation of Human Mesenchymal Stem Cells in Freeze-Gelled Chitosan/Nano ẞ-tricalcium Phosphate Porous Scaffolds Crosslinked with Genipin,” Materials Science and Engineering C, Vol, 54, pp. 76-83, 2015. [20] W. Thein, R. Misra, “Biomimetic ChitosanNanohydroxyapatie Composite Scaffolds for Bone Tissue Engineering,” Acta Biomaterialia, Vol, 5, pp. 1182–1197, 2019. [21] W. Wattanutchariya, W. Changkowchai, “Characterization of Porous Scaffold from Chitosan-Gelatin/Hydroxyapatite for Bone Grafting,” Proceedings of the International MultiConference of Engineers and Computer Scientists, Vol II, 2014. [22] S. Pineda-Castillo, A. Bernal-Ballén, C. Bernal-López, H. Segura-Puello, D. NietoMosquera, A. Villamil-Ballesteros, L. Munster “Synthesis and Characterization of Poly(Vinyl Alcohol)-Chitosan-Hydroxyapatite Scaffolds: A Promising Alternative for Bone Tissue Regeneration,” Molecules, Vol. 23, pp. 2414, 2018. | ||
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