DNA Fingerprints of Two Tilapia Fish Species of Euphrates River at Governorate ofAl-Muthanna Using RAPD Markers | ||
Engineering and Technology Journal | ||
Article 20, Volume 37, 3C, June 2019, Pages 345-349 PDF (654.58 K) | ||
Document Type: Research Paper | ||
DOI: 10.30684/etj.37.3C.7 | ||
Authors | ||
Taha Al-Khafaji1; Mustafa S.F. Ziyadi2; Marwa K. Musad3 | ||
1College of Agriculture, University of Muthanna Dept. Animal Production Al-Muthanna -Iraq | ||
2Marine Science Centre, University of Basrah Dept. Marine Vertebrate Basra -Iraq | ||
3college of Agriculture, University of Muthanna Dept. Animal Production Al-Muthanna -Iraq | ||
Abstract | ||
During the last decade, tilapia species (Cichlidae: Teleostei) became members of Iraqi fish fauna. They characterized with morphological similarity among genera and species. That makes species differentiation not easily. Molecular methods followed to differentiate between redbelly tilapia Coptodon zillii (Gervais, 1848) and blue tilapia Oreochromis aureus (Steindachner, 1864) of the Euphrates River sector at Al-Samawah city. RAPD-PCR method used to create the genetic fingerprints of two tilapia fish species. Seven decamer primers (OPA08, OPA10, OPA13, OPA17, OPA19, OPB08 and OPC02) used to amplify DNA fragments using PCR-RAPD technique. Forty-four bands scored after electrophoresis on 2% agarose gel along with molecular marker fragmented to each 100 base pair. The molecular weight of bands was calculated using PhotoCapt-MW software. The volume of bands ranged from C. zillii 168 bp to 2227 bp while they ranged from 62 bp to 2154 bp in O. aureus. The results achieve the RAPD fingerprints of two tilapia species in Euphrates River at for genetic Al-Samawa city and draw the genetic tree with the same species from Shatt A-Arab River in Basrah city. The study concluded that there is the closest relatedness among tilapia populations from Euphrates and Shatt Al-Arab Rivers. The results proved that RAPD markers were efficient to generate DNA fingerprints of tilapia fish species. Furthermore, the utilizing of the RAPD markers can differentiate the two studied species. The present study may be the first genetic study on these tilapia fish species. Moreover, this would be the baseline studies in the future. In addition, this study would be valuable for conservation program and documentation of identities of tilapia fish species in Iraqi inland waters | ||
Keywords | ||
DNA; Fingerprints; PCR; RAPD; Tilapia; Iraq; Euphrates | ||
References | ||
[1] A.H. J. Abdullah, R. A.K. Faris and S. A. Abdullah, “Structural Diversity of Fish Assemblage in the Southern Sector of Main Outfall Drains northwest of Basrah, Iraq,” Basrah J. Agric. Sci., Vol. 31, No. 1, pp. 1-11, 2018. [2] Kh. E. Saleh, “New record of Tilapia zilli in Iraqi natural waters in Euphrates River” 1 st Scientific Conference of Agriculture, College of AgricultureUniversity of Basra. (Arabic abstract), 2007. [3] F.M. Mutlak, and A.J. Al-Faisal, “New record of two exotic cichlid fish Oreochromis aureus (Steindachner, 1864) and Tilapia zilli (Gervais, 1848) from south of the main outfall drain in Basrah city,” Mesop. J. Mar. Sci., Vol. 24, No. 2, pp. 160 – 170, 2009. [4] A.J. Al-Faisal, and F.M. Mutlak, “First record of the Nile tilapia Oreochromis niloticus (Linnaeus, 1758), from the Shatt Al-Arab River, Southern Iraq,” International Journal of Marine Science, Vol. 5, No. 38, pp. 1-3, 2014. [5] J. De Maeseneer, “The culture of Tilapia species in tropical and subtropical conditions,” Tropiculture, Vol. 2, No. 1, pp. 19-25, 1984. [6] FAO. “Aquaculture Production, Year Book of Fishery Statistics,” - Vol. 96/2. Food and Agriculture Organization of the United Nations, Rome, Italy, 125 p., 2005. http://www.fao.org/fishery/publications/yearbo oks/en. [7] C.W. Martin, M.W. Valentine, J.F. Valentine, “Competitive Interactions between Invasive Nile Tilapia and Native Fish: The Potential for Altered Trophic Exchange and Modification of Food Webs,” PLoS ONE, Vol. 5, No. 12, pp. e14395, 2010. [8] P. Sodsuk, and B.J. McAndrew, “Molecular systematics of three tilapia genera Tilapia, Sarotherodon and Oreochromis using allozyme data,” J. of Fish Biol., Vol. 39, pp., 301-308, 1991. [9] S. Seyoum, “Allozyme variation in subspecies of Oreochromis niloticus (Pisces: Cichlidae),” Isozyme Bull., Vol. 23, pp., 97, 1990. [10] J.F. Agnese, B., Adépo-Gourène, E.K. Abban, and Y. Fermon, “Genetic differentiation among natural populations of the Nile tilapia Oreochromis niloticus (Teleostei, Cichlidae),” Heredity, Vol. 79, pp. 88–96, 1997. [11] M. Lynch, and B.G. Milligan, “Analysis of population genetic structure with RAPD markers,” Molecular Ecology, Vol. 3, pp. 91-99, 1994. [12] J. Welsh, and M. McClelland, “Fingerprinting genomes using PCR with arbitrary primers,” Nucleic Acids Research, Vol. 18, No. 24, pp. 723-7218, 1990. [13] J.G.K. Williams, A.R. Kubelik, K.J. Livak, J.A. Rafalski, and S.V. Tingey, “DNA polymorphisms amplified by arbitrary primers are useful as genetic markers,” Nucleic Acids Research, Vol. 18, No. 22, pp. 6531-6535, 1990. [14] H. Hadrys, M. Balick, and B. Schierwater, “Applications of random amplified polymorphic DNA (RAPD) in molecular ecology,” Mol. Ecol., Vol. 1, pp. 55- 63, 1992. [15] F. Bardakci, and D.O.F. Skibinski, “Application of RAPD technique in tilapia fish: species and subspecies identification,” Heredity, Vol. 73, pp. 117-123, 1994. [16] K.R., Dinesh, T.M. Lim, W.K. Chan, and V.P.E. Phang, “Genetic variation inferred from RAPD fingerprinting in three species of tilapia,” Aquaculture International, Vol. 4, 19-30, 1996. [17] M.M.M. Ahmed, B.A. Ali, and S.Y. EI-Zaeem, “Application of RAPD markers in fish: Part I – some genera (Tilapia, Sarotherodon, and Oreochromis) and species (Oreochromis aureus and Oreochromis niloticus) of Tilapia,” Int. J. Biotechnology, Vol. 6, No. 1, pp.86–93, 2004. [18] C. Callejas, and M.D. Ochando, “Identification of Spanish barbell species using the RAPD technique,” J. Fish Biol. Vol. 53, pp. 208-215, 1998. [19] C. Callejas, and M.D. Ochando, “Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the Iberian Peninsula,” J. Fish Biol., Vol. 59, pp. 1589-1599, 2001. [20] C. Callejas, and M.D. Ochando, “Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers,” Heredity, Vol. 89:36-43, 2002. [21] Z. Mamuris, C. Stamatis, and C. Triantaphyllidis, “Intraspecific genetic variation of striped red mullet (Mullus surmuletus L.) in the Mediterranean Sea assessed by allozyme and random amplified polymorphic DNA (RAPD) analysis,” Heredity, Vol. 83, pp. 30-38, 1999. [22] J-M. Yoon and J-W., Kim “Randomly amplified polymorphic DNA-polymerase chain reaction analysis of two different populations of cultured Korean catfish Silurus asotus.” J. Biosciences, Vol. 26, No. 5, pp. 641- 647., 2001. [23] D. Jini, “Genotypic Analysis of Etroplus Maculatus in Western Ghat Rivers of India using Random Amplification of Polymorphic DNA (RAPD) analysis,” J. of Genetics and Genetic Engineering Vol. 1, No. 1, PP. 1-8, 2017. [24] M.S. Faddagh, N.A. Husain and A.I. Al-Badran, “DNA fingerprinting of eight cyprinid fish species of Iraqi inland waters using RAPD-PCR,” Advances in Life Sciences, Vol. 2, No. 2, pp. 9-16, 2012. [25] M.S. Faddagh, “Usage of new decamer primer MB1- 9 to discriminate six Barbus fish species (Cyprinidae: Teleostei) in Iraqi freshwaters,” Basrah J. Research Sciences, Vol. 40, No. 3, Part A, pp. 148-155, 2014. [26] M.S. Faddagh, “Genetic Diversity of Kattan Luciobarbus xanthopterus Heckel, 1843 (Pisces: Cyprinidae) in Four Mesopotamian Inland Waters, Iraq,” Basrah J. Agric. Sci., Vol. 29, No. 1, pp, 25-33, 2016. [27] A.J. Al-Faisal, A.R. M. Mohamed, T. A. Jaayid, “New record of the carangid fish from the Iraqi marine waters, northwest Arabian Gulf” Mesop. Environ. J., special issue A, pp. 106-114, 2016. [28] FishBase, “Fishbase.org/ Identification Keys, 2019 [online]. https://www.fishbase.se/keys/allkeys.php. [29] UPGMA, “Unweighted Pair Group Method with Arithmetic Mean [online]. http://genomes.urv.cat/UPGMA/, 2016. [30] MS. Faddagh, T.Y. Al-Khafaji, and M.K. Musaad, “DNA fingerprints of Tilapia species in Shatt Al-Arab River using RAPD markers,” Unpublished. [31] J. Briolay, N. Galtier, R.M. Brito and Y. Bouvet “Molecular phylogeny of Cyprinidae inferred from cytochrome b DNA sequences,” Mol. Phylogenet. Evol., Vol. 9, No. 1, pp. 100-108, 1998. [32] A. Machordom, I. Doadrio, and P. Berrebi, “Phylogeny and evolution of the genus Barbus in the Iberian Peninsula as revealed by allozyme electrophoresis” J. Fish Biol., Vol. 47, No. 2, pp. 211-236, 1995. [33] O.H.M. Shair, R.M. Al- Ssum, A.H. Bahkali, “Genetic variation investigation of tilapia grown under Saudi Arabian controlled environment,” Am. J. Biochem. Mol. Biol, Vol. 1, pp. 89-94, 2011. [34] B. Neekhra, A.A. Mansoori, S. Verma, R.K. Koiri and S.K. Jain, “RAPD-PCR Based Biomarker Study in Fish Species (Family: Cyprinidae) of Madhya Pradesh, India,” Austin J. Mol & Cell Biol. Vol. 1, No. 1, pp. 1003, 2014. | ||
Statistics Article View: 221 PDF Download: 294 |