Application of species-specific polymerase chain reaction technique depending on cytochrome b gene for beef authentication | ||
| Iraqi Journal of Veterinary Sciences | ||
| Article 3, Volume 26, Suppl. III - Serial Number 4, September 2012, Pages 193-196 PDF (195.51 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/ijvs.2012.168695 | ||
| Authors | ||
| E.A. Younis; R.A. Al-Sanjary | ||
| Department of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, Iraq | ||
| Abstract | ||
| The study was conducted on imported beef meat (Indian, Brazilian and Australian), and spacement of minced beef meat (Al-jnobi, Al-sharabi) and Al-frezian collected from local grocery stores for beef authentication to differentiate them from others meats in order to protect consumer economically through applying Species-specific Polymerase Chain Reaction technique using n cytochrome b gene. Results of this study indicated that Species-specific PCR technique was very sensitive and highly specific for the identification of the meat type, also it was found that the designed primer on mitochondrial cyt b gene of beef proved practically proved to be applied on local and imported types of meat of different breeds, since gives the same molecular weight 365 bp in all the same bands. From five minced beef samples, one sample was pure beef, other one contained no beef at all, while the remaining three samples were mixed meat of beef, buffalo, goat and mutton. | ||
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