EVALUATION OF GENETIC VARIABILITY BETWEEN THREE LINES OF CHICKENS BASED ON RAPD-PCR AND 18S rRNA GENE SEQUENCING IN ERBIL (IRAQI KURDISTAN REGION) | ||
| Mesopotamia Journal of Agriculture | ||
| Article 2, Volume 49, Issue 3, September 2021, Pages 12-26 PDF (831.29 K) | ||
| Document Type: Research | ||
| DOI: 10.33899/magrj.2021.129979.1129 | ||
| Authors | ||
| Hemin Hussein Ali Omarbly* 1; Dilger Maghded Khdr1; Rozhgar A. Khailany2; Aram M. Ahmed3; Shirkoo Ameen Fateh Salai4 | ||
| 1Department of Animal Resources, College of Agricultural Engineering Sciences, Salahaddin University- Erbil, Kurdistan Region, Iraq | ||
| 2Department of Biology, College of Science, Salahaddin University- Erbil, Kurdistan Region, Iraq | ||
| 3Department of Animal Science, Directorate of Agricultural Research- Erbil, Ministry of Agriculture and Water Resources, Kurdistan Region, Iraq | ||
| 4Department of Plant Protection, Directorate of Agricultural Research- Erbil, Ministry of Agriculture and Water Resources, Kurdistan Region, Iraq | ||
| Abstract | ||
| The current study was carried out to evaluate and identify the genetic variation between Local Black chicken lines with two commercial Layer (Isa Brown) and Broiler (Rose) breeds using RAPD markers and sequencing approach of 18s rRNA gene. From the result of the RAPD marker, all primers used were produced 152 scorable bands ranging from 2 to 9 with the size ranging from 320 to 2990 bp with percentage polymorphic loci 64.86% among chicken populations. The highest amplified fragments by primer OPC-11 and lowest by OPAA-03 were 24 and 8, respectively. The mean of the observed number of alleles (na), effective number of alleles (ne), gene diversity (h), Shannon's information index (I) for all loci found to be 1.6486, 1.5189, 0.2883 and 0.4129, respectively. The existence of a high level of polymorphisms and targeted (74) loci throughout all chicken populations/primers indicate sufficient genetic distance and more genetic variability among chicken populations using RAPD-PCR techniques. Result of blasted sequences of 18srRNA gene of local chicken has GenBank accession number MT808178 and MT808179 by BLAST tool against Gallus gallus, it showed the highest identity 95.74% and 94.88% for data of first and second part, respectively. The overall dendrograms clustered showed that the local chicken was closer to the commercial layer than to the broiler chicken lines. Therefore, it suggests that improving the local Black chicken line according to the layer breeding program to collect genetically invaluable genetic resources | ||
| Keywords | ||
| Dendrogram,,; ,،,؛Genetic diversity,,; ,،,؛Local Black chicken,,; ,،,؛Molecular marker | ||
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