Molecular Identification of Local Isolated Streptomyces Species from North Region soil in Iraq | ||
| Journal of Education and Science | ||
| Article 5, Volume 30, Issue 3, August 2021, Pages 45-62 PDF (2.08 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/edusj.2021.168654 | ||
| Authors | ||
| Omer F. Ghazi; Safa I. Al-Obaidi | ||
| Department of biology, college of education for pure sciences, Mosul University, Mosul, Iraq | ||
| Abstract | ||
| In this study Streptomyces were isolated from 50 bacterial isolates taken from 30 soil samples, these samples were collected from various locations in Iraq's various regions. The species of Streptomyces were isolated using starch casein agar and diagnosed microscopically and morphologically by Gram staining and glass slide. The sequence analysis 16S rRNA is used to report 11 Streptomyces. 10 bands of DNA gene, a result of specific polymerase chain reaction PCR, are elected from bacterial local isolates where 1000 base pairs within one volume, The PCR products of DNA samples were chosen from 11 local isolates based on nitrogenous base sequences. These organisms are revealed as a result of the study and by using DNA Blast NCBI as fellows; Streptomyces gancidicus, S. werraensis, S. griseorubens, S. hawaiiensis, S. thermocarboxydus, S. cyaneus, S. misionensis, S. bellus, S. parvulus, S. labedae, | ||
| Keywords | ||
| Streptomyces. specific PCR; Sequencing analysis | ||
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