Effect of Lipopolysaccharide Extracted from Enterobacter cloacae on some Pathogenic Bacteria | ||
| Rafidain Journal of Science | ||
| Article 2, Volume 29, Issue 4, December 2020, Pages 8-13 PDF (432.32 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/rjs.2020.167308 | ||
| Authors | ||
| Rawaa Ahmed AL Aswad* 1; Hiyam Adel Altaii2 | ||
| 1university of Mosul | ||
| 2Biology College of Science University of Mosul Mosul/Iraq | ||
| Abstract | ||
| A 106 different specimen were collected to isolate and diagnose some gram negative and gram-positive bacteria, as it was performed for the period from 15 August to 30 November 2019 from AL-Salam and Al-Khansaa educational hospitals in Mosul. Lipopolysaccharide (LPS) was extracted from Enterobacter cloacae by using the Methanol - Chloroform method. extract was diagnosed, using some biochemical tests such as the Molisch test to detect carbohydrate content and Folin reagent in order to determine proteins concentration, lipid solubility in organic solvent and Bio drop technique as well as to detect nucleic acid concentration. The extraction methods showed its efficiency with low protein and nucleic acids concentration in the extract. Lipopolysaccharide demonstrated its efficiency inhibiting microbial growth on all test isolates at concentrations 1100, 1000 µg\ ml while its lipopolysaccharide showed its inhibition of Proteus mirabilis at all concentrations used. LPS showed possibility used as inhibitory, antimicrobial growth | ||
| Keywords | ||
| Outer membrane; Inhibiting effect; extraction; Lipopolysaccharide | ||
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