Prevalence and molecular identification of Cryptosporidium spp in cattle in Baghdad province, Iraq | ||
Iraqi Journal of Veterinary Sciences | ||
Article 32, Volume 33, Issue 2, September 2019, Pages 389-394 PDF (305.02 K) | ||
Document Type: Research Paper | ||
DOI: 10.33899/ijvs.2019.163084 | ||
Authors | ||
H.H. Alseady; M.H. Kawan | ||
Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq | ||
Abstract | ||
The objective of this study was to record the prevalence rate of Cryptosporidium and to determine the Cryptosporidium spp in cattle in different parts of Baghdad province. A total number of 100 fecal samples from different age groups were collected. Conventional method using modified Ziehl-Neelsen for staining fecal smears and molecular techniques for detection the prevalence and determines the species of Cryptosporidium that cause infection. The overall prevalence of infection with Cryptosporidium was 21% (21/100) by conventional method; nested PCR was done that targeting 18S rRNA gene on the same samples in which Cryptosporidium DNA identified in 38 samples (38%). Four species of Cryptosporidium in cattle were detected for the first time in Baghdad province: C. parvum (6/10), C. andersoni (2/10), C. bovis (1/10) and C. ryanae (1/10). The determination and characterization of Cryptosporidium spp in cattle was very important to avoid the infection to other animals and handlers and for applying control programs. | ||
Keywords | ||
<i>Cryptosporidium</i>; prevalence; cattle; Nested PCR; Sequences analysis | ||
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