spectrophotometric determination of l-arginine and lysine using a red lysarine reagent S In aqueous solution | ||
| Journal of Education and Science | ||
| Article 12, Volume 26, Issue 1, March 2013, Pages 92-102 PDF (733.51 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/edusj.2013.162846 | ||
| Authors | ||
| Thabit ٍSaeed Alghabsha* ; Amal Mohammad Saeed* | ||
| Abstract | ||
| A simple, rapid and selective spectrophotometric method for the determination of arginine and lysine was developed. The method is based on the proton transfer reaction of arginine and lysine with alizarin red sulphonate in aqueous solution to form a violet coloured product which has maximum absorption at 530 nm. Beer’s law obeyed over the range (1-10), (1-11) mg.ml-1 for arginine and lysine respectively with a molar absorptivity 7830 l.mol-1.cm-1 for arginine and 5990 l.mol-1.cm-1 for lysine and accuracy (average recovery) 100.63% and precision (RSD) is less than 2.5%. The method has been applied for the determination of arginine in urine. | ||
| Keywords | ||
| Spectrophotometric determination; L-Arginine; lysine; red lysarine | ||
|
Statistics Article View: 325 PDF Download: 299 |
||