MOLECULAR IDENTIFICATION OF GRAPEVINE FANLEAF VIRUS | ||
Mesopotamia Journal of Agriculture | ||
Article 24, Volume 45, Issue 3, October 2017, Pages 271-274 PDF (332.7 K) | ||
Document Type: Research | ||
DOI: 10.33899/magrj.2019.161355 | ||
Authors | ||
Firas Al Juboori,* 1; N. A. Qassem,1; , M. Al-Maadhedi2 | ||
1Plant protection Dept., College of Agriculture and Forestry, Mosul University. Iraq. | ||
2Ministry of Agriculture Iraq | ||
Abstract | ||
This study was conducted to molecular diagnostics of Grapevine fanleaf virus (GFLV) in leaf samples of grapevine were collected from the grapevine yards in Salahuddin governorate, where grapevines are commonly grown in Iraq. The virus was detected in the samples by using SuperScriptTM III RT kit and pair of specific primers GFLV2231F and GFLV2533R was used to amplify fragments of the coat protein gene (CP) in rapid direct one tube RT-PCR. | ||
Keywords | ||
Grapevine fanleaf virus; GFLV; RT-PCR; Iraq | ||
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