Morphological and Molecular Diagnosis of Root Knot Nematode Species Associated with Olive Seedling and Trees in Baghdad, Babil and Karbala/ Iraq | ||
| Rafidain Journal of Science | ||
| Article 27, Volume 27, Issue 4, December 2018, Pages 138-150 PDF (1.06 M) | ||
| DOI: 10.33899/rjs.2018.159350 | ||
| Authors | ||
| Waref M. Hanoon* 1; Zeinab A. Altememe2; Asmaa M. Abdel rasool3 | ||
| 1Department of Plant Protection, College of Agriculture, University of Karbala | ||
| 2Department of Plant Protection, College of Agriculture ,University of Karbala | ||
| 3Department of Plant Protection, College of Agriculture and Forestry, University of Mosul | ||
| Abstract | ||
| The study was aimed to conduct a field survey to identify the Root knot nematodes species associated with olive trees and nurseries in provinces of Baghdad /Al- Zaafaraniya, Babil /Al- Sada, Karbala /Al-Atishi and Al-Hindiah Horticulture Center. Baghdad /Al- Zaafaraniya had the highest percentage of disease incidence (100%) and root-knot index (3) in the field, while nurseries under shade conditions in the same region had the least percentage of disease incidence and root-knot index (6.6% and 0), respectively. Two species of root-knot nematodes M. javanica and M.incognita were identified in all study location, the perineal pattren technique was used for identification .M.incognita identification was molecularly confirmed by the PCR to be used in all study trials. | ||
| Keywords | ||
| olive; Root knot nematode Diagnosis; Perineal Pattran; PCR | ||
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