Serological and Molecular Comparison Study for Diagnosis of Cytomegalovirus Infection in aborted Pregnant Women in Iraq | ||
| Rafidain Journal of Science | ||
| Article 10, Volume 27, Issue 4, December 2018, Pages 81-89 PDF (622.51 K) | ||
| DOI: 10.33899/rjs.2018.159336 | ||
| Authors | ||
| Anmar A. Al-Taie* 1; Basim A. Abdullah2; Mozahim Y. Al-Attar3 | ||
| 1Department of Biology,College of Science, University of Mosul | ||
| 2Deptartment of Biology,College of Science,University of Mosul | ||
| 3Department of Microbiology, College of Medicine, University of Mosul | ||
| Abstract | ||
| This study was conducted to investigate the Cytomegalovirus (CMV) infections of pregnant women. The samples were collected from Mosul and Baghdad hospitals in Iraq for two years and the tested women within the age category of under 20 to above 39 years. One thousand five hundred samples were taken as serum, to use in ELISA (IgM, IgG). EDTA blood, Heparin blood and cervical swabs were used in molecular tests. Three hundred positive samples demonstrating the presence of IgM and IgG antibodies using ELISA test. IgM antibodies were positive in 146 (48.7%) CMV, IgG antibodies were positive in 189 (63%) for CMV. DNA was extracted and Real-Time PCR indicates positive in only four samples (1.3%) in CMV from all 300 positive samples in ELISA tests. CMV | ||
| Keywords | ||
| CMV Infections; ELISA for CMV; Real; time PCR | ||
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