MUTAGENESIS OF A Pseudomonos aeruginosa By USING NITROUS ACID | ||
| Journal of University of Anbar for Pure Science | ||
| Article 11, Volume 1, Issue 3, December 2007, Pages 45-49 PDF (410.28 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2007.15654 | ||
| Author | ||
| Khalaf Jasim Mohammed Al-Dolaimi* | ||
| Department of Biology - College of Science - University of Anbar | ||
| Abstract | ||
| The bacterium Pseudomonas aeruginosa was isolated from patients of burns in Al-Ramadi hospital and diagnosed according to its cultural , microscopic and biochemical properties. sensitivity test of the bacterium against antibiotics was established and the results appeared it was sensitive to the antibiotics Carbenicillin , Chloramphenicol , Rifampicin and Trimethoprim and resistant to the antibitcs Amoxicillin , Ampicillin Clindomycin Cloxacillin , Nalidixic acid , Cephaloxin , Tetracyclin and Tobromycin . Results of mutagenesis showed resistance of the bacterium to the lethal effect of nitrous acid and its sensitivity to the mutagenic effect of the acid relative to the antibiotics Rifampicin and Chloramphenicol but not to the antibiotics Carbenicillin and Trimethoprim . | ||
| Keywords | ||
| Pseudomonas aeruginosa | ||
| References | ||
|
]1[Auerbach , c. (1962) . Mutation ,an introdution to research on mutagenesis . Oliver and Boyed , Edinburgh .
]2[Shortle , D . D . , Maio , D . and Nathans , D. (1981) . Directed Mutagenesis . Ann.Rev.Biochem . 15:265-294.
]3[Singer , B.and Kusmierek , J . T . (1982). chemical mutagenesis . Ann.Rev.Biochem. 51: 655 – 693 .
[4]Al- Habib , H.M. and Al-Talib , A.B. (1985). pseudomonas aeruginosa isolated from four types of infections and its sensitivity to antibiotics . journal of faculty of Medicin (Baghdad) 27: 35-43
[5]Al-Bakri,G.H.and Umran , M.A. (1994) Mutagenesis of a novel halotolerant bacteria (Micrococcus SP.) using ultraviolet light and N-Methlyl –N-nitro-N-Nitroso- guanidine Iraqi Journal of Microbiology , vol. 6(2): 55-64 .
]6[Bowring , S.N. and Morris , J . C . (1985) – Mutagenesis of Clostridium acetobutylicum . J . APPI . Bacteriol . 58:577-584.
]7[Kerszman , G . (1975) . Induction of mutation to Streptomycin resistance in Micrococcus radiodurans . Mutation Res . 28: 9-14 .
]8[Frifelder , D . (1987) . Molecular biology – 2nd . Yones and Barttett Boston . | ||
|
Statistics Article View: 119 PDF Download: 120 |
||