The Role of Lactate Dehydrogenase in Differentiating Between Bacterial and Non -Bacterial Meningitis in children Under Five Years in Ramadi Maternity and Children Hospital | ||
| Journal of University of Anbar for Pure Science | ||
| Article 1, Volume 3, Issue 3, December 2009, Pages 1-5 PDF (273.76 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2009.15592 | ||
| Authors | ||
| Adnan M Jasim* 1; Muhammad M. Mushrif2; Saleem O. Qateae3 | ||
| 1college of medicine – university of Al- Anbar | ||
| 2college of medicine –university of al- Anbar | ||
| 3Ministry of health – Al-Anbar general health directorate | ||
| Abstract | ||
| The present study was designed to find out the usefulness of lactate dehydrogenase (LDH) level in cerebrospinal fluid (CSF) in differentiating bacterial from non-bacterial meningitis in children below 5 year of age . A total of 177 patients who were admitted to the AL-Ramadi Maternity and children hospital, AL-Anbar, Iraq During a period of one year ( from 1st.November / 2007 to 1st.November 2008), for diagnosis and treatment of meningitis were included in the study. Cerebrospinal fluid samples were collected from patients by lumbar puncture(LP) for diagnosis. Levels of LDH were measured spectrophotometrically in Randox commercially prepared kits and compared in bacterial , non-bacterial meningitis and controls.The study revealed that only 24 cases have meningitis. Bacterial meningitis present in 19(79%) . Of them 11(46%) with positive culture and 8(33%) with negative culture (partially treated), while the remaining 5(21%) were found to be non-bacterial cases. The study concluded that measurement of LDH levels in CSF is beneficial in differentiating bacterial from non-bacterial meningitis since it was significantly increased in bacterial meningitis. | ||
| Keywords | ||
| lactate dehydrogenase; Meningitis; ramadi | ||
| References | ||
|
1. Swartz M.N. Bacterial meningitis in: Cecil textbook of Medicine 21st EdGoldman and Bennett. W.B. Saunders Company: 1644 – 1649.
2. Dawson K.G., Emerson J.C., Burns J.L. Fifteen years of experience with bacterial meningitis. Pediatric. Infect. Dis. J. 18(9) :816 - 822 .
3. Wenger JD , Hightower AD , Facklam RR , Gaventa S , et al. the Bacterial meningitis study group ; Bacterial meningitis in the , United states , 1986 : report of a multistate surveillance study . Journal of infectious diseases (1990) 162;1316 – 1323.
4. Types and causes of meningitis, meningitis research foundation office, England and Wales 2007.
5. Amir Vokshoor Viral meningitis: Article by MD, Staff, Department of neurosurgery, Woodland Hills, medical Center, January 9, 2004.
6. Bonadio WA. The cerebrospinal fluid: physiologic aspect and alteration associated with bacterial meningitis. Pediatr infect Dis J 1992; 11:423-32.
7. Colle J.G., Duguid J.P.; Fraser A.G.; Marmion B.P, et al. laboratory Strategy in the diagnosis of infective syndromes. Churchill & Livingstone,14:35-94.
8. Charles G. Prober. Central nervous system infection .Nelson textbook of pediatrics, Kliegman, Behrman, Jenson, Stanton. Philadelphia18th edition 2007:2513.
9- Fleisher M, Wasserstrom WR,Schold SC, Schwartz MK, posner JB.Lactic dehydrogenase isoenzymes in the cerebrospinal fluid of patients with systemic cancer. Cancer 1981;47:2654-9.
10- Subhash MN, Rao BSS, Shankar SK. Changes in lactate dehydrogenase isoenzymes pattern in patients with tumors of the central nevous system. Neurochem Int 1993;22:121-4.
11. McArthur JC, Nance-Sproson te, Griffin de, et al. The diagnosis utility of elevation in cerebrospinal fluid beta 2-microglobulin in HIV-1 dementia.Neurology 1992;42:1707-12 .
12. Watson MA, Scott MG.Clinical utility of biochemical analysis of cerebrospinal fluid. Clin Chem1995;343-60.
13. J. A. Knight, S. M. Dudek, and R. E. Haymon Early (Chemical) Diagnosis ofBacterial Meningitis. Cerebrospinal Fluid Glucose, Lactate, and Lactate Dehydrogenase Compared. clin . Chem. 27(8): 1431-1434(1981).
14. Andreas Wellmer, Juliane Prange, Joachim Gerber, Gregor Zysk, et al. D-and L-lactate in Rabbit and Human Bacterial meningitis. Scandinavian Journal of infectious Diseases December 2001;33:909-913.
15-Coupal L, Suissa S, Szentveri T, Falutz J, et al .The clinical utility of serum lactate dehydrogenase in diagnosing pneumocystis carinii pneumonia among hospitalized AIDS patients.Clin invest med. 1992;15(4): 309-17.
16- Abdul Khaliq A. Sallam, Etilogy and presentation of Acute bacterial Meningitis in children at Al-Thawrah hospital, sanaa, yemen.
17- Converse, G. M., Gwalteny, j. M., Strassburg, D.A., and Hendley, j. O. (1973). Alteration of cerebrospinal fluid finding by partial treatment of bacterial meningitis. Journal of pediatrics, 83,220.
18- Jarvis, C. W., and Saxena, K. M. (1972). Does prior antibiotic treatment hamper the diagnosis of acute bacterial meningitis .An analysis of a series of 135 childhood cases. Clinical pediatrics, 11, 201.
19 – Al-Mawla S.O.G. A Study on the aetiology of bacterial meningitis and pre purposes.PhD Thesis. Al-Mustensiriyah University, College of Medicine 2002: P 51.
20- Izidore S. Losses, M .D.,cerebrospinal fluid lactate dehydrogenase isoenzyme Analysis for the,Hadassah university hospital December 1999;52:198.
21- Koul R.L.; Chacko A.(1994). Pattern of childhood meningitis in Oman. Saudi. Med. J. 19(4): 432-436.
22- Rao B.N.; Kashbur I.M.; Shembesh N.M. and El-Bargathy S.M. (1998). Aetiology and occurrence of a in Benghazi, Labyan Arab Jamahiriyia. East. Meditterr. Health.J.4 (1): 50-57.
23- Murray CJ and Lopez AD. Global Burden of Disease and Injury Series Vol II. Global Health Statistics. Geneva, 1996:285.
24- Baraff LJ,Lee SI and Schriger DL. Outcomes of bacterial meningitis in children: a meta-analysis. Pediatr Infect Dis J 1993; 12:389-94.
25- Quagliarello VJ, Scheld WM. Treatment of bacterial meningitis. N Engl J Med. 1997;336: 708 -16.
26- Al-Aun M.; Saleh F.; Ajlouni M.B.; Hazza I, et al. Bacterial Meningitis in children. J. Roy.Med. Serv 1994;1(2): 23-25.
27- Mayo Foundation for Medical Education and Research by Mayo Clinic Staff, infectious disease, meningitis; 11, 2007
28- Schuchat A., Wenger J. Epidemiology of bacterial meningitis. Annual Nestle 1997;55: 79-91.
29- Gastrin, B., Briem, H., and Rombo, Rapid diagnosis of meningitis with use of selected clinical data and gas-liquid chromatographic determination of lactate concentration in cerebrospinal fluid. J. Infect.Dis. (1979); 139:529-533 .
30- William Neches M.D.and Martin PlattM.D. cerebrospinal fluid LDH in 287 children, including 53 cases of meningitis of bacterial and non-bacterial etiology. Pediatrics1986;41(6):1097-1103.
31- W. E. Feldman, hillock. cerebrospinal fluid LDH activity. Clin pediatric 1978;17:372-375.
32- Krogsgaard AR,Ouaade. Glutamic acid oxaloacetic acid system. Acta Neurol Scand 1963;39:154-60.
33- Nussinovitch M, Klinger G, Magazanik A, Volovitz B, et al. increase creatine kinase brain isoenzyme concentration in cerebrospinal fluid with meningitis. Clin pediatr(phila)1996;35:349-51.
34- AKimor GA, Zinccheno VA, Sytinsky IA, palagnta VD. The gamma glutamyl transferase activity in hemorrhagic and ischemic stroke. Sov Med 1985;1:3-5 .
35- pagana, Kathleen Deska. Mosby's Manual of diagnostic and laboratory Tests. St Louis: mosby, inc.,1998.
36- Connolly K, Hammer SM. The acute aseptic meningitis syndrome. Infect Dis Clin North Am 1990;4:599-622.
37- Nelson S, Sealy DP, Schneider EF. The acute aseptic meningitis syndrome. Am Fam Physician 1993;48:809-15.
38- B. S. Nayak and Rama Bahat. Cerebrospinal fluidlactate dehydrogenase and glutamine in meningitis. Department of Biochemistry and medicine kasturba medical college 2005; 49(1):108-110.
39- Beaty HN,Oppenheimer S;Cerebrospinal fluid lactic dehydrogenase and its isoenzymes in infection of the central nerveous system. New Eng J Med 1988;279:1197-1202.
40- Khanna SK, Gupta DK, Gupta BK, Khanna P: Cerebrospinal fluid lactic dehydrogenase activity in various neurological disorders J Assooc phys Ind 1976;24:29-36. | ||
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