EXTRACTION AND IDENTIFICATION OF ATROPIN FROM DATURA INNOXIA AND STUDYING EFFECT ON SOME ENZYMES. | ||
| Journal of University of Anbar for Pure Science | ||
| Article 33, Volume 4, Issue 1, April 2010, Pages 116-126 PDF (590.89 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2010.15508 | ||
| Authors | ||
| FIRAS T.MAHER1; AYAD S. HAMMED* 2; TAPPAN K.KAKAH1 | ||
| 1Tikrit University - College of Science | ||
| 2Tikrit University - College of Education, Iraq | ||
| Abstract | ||
| The work included extraction and identification of atropine.The alkaloid of atropine was extracted from Datura Innoxia using water extraction method.The purification of atropine was accomplished using several chromatographic Thin Layer chromatography (TLC),HPLC method and spectroscopic methods (UV,IR) The results obtained above are compatable with atropine structural formula.The enzymetic activity study of atropine extract have been carried out against acid phosphatase enzyme (ACP), the result obtained medicated that شtropine showed good activition against (ACP) enzyme. The presence of atropine extract showed lowering the concentration of Uric acid , Urea and Sugar in the blood serum. Finlly,the atropine extracted was evaluated for its antibacterial activity against Gram positive (G+) (Staphylococcus aureus,Lactobacillus) and Gram negative (G-) (Escherechia Coli , Proteusral), the result obtained indicated that atropine extract exhibited reasonable antibacterial activity | ||
| References | ||
|
1-Chakravatrty,H.L.(1976).Plant wealth of Iraq. Dictionary of Economic plants vol.I, Botany Directorate. Minstry of Agriculture and Agrian Reform .Iraq.
2- Al,Rawi,A.,1966 Poisonous plants in Iraq. Ministry Agriculture P.65.
3-Chakravatrty,H.L.(1976).Plant wealth of Iraq. Dictionary of Economic plants vol.I, Botany Directorate. Minstry of Agriculture and Agrian Reform .Iraq.
4-الخالدی,مؤید صبری شوکت (2005) انتاج بعض القلویدات من نوعین من نبات الداتورة Datura stramoniumوDatura anoxia بأستعمال تقنیة زراعة الأنسجة النباتیة, أطروحة دکتوراه, کلیة العلوم, جامعة بغداد 5- القطب,فوزی طه (1985).النباتات الطبیة وزراعتها ومکوناتها. دار المریخ للنشر الریاض. 5- Jasperon,Rita J. Schit.(1997).Acute poionigs with toxic plants in switzerl and between 1960 and 1994.126:1085-1095.peer reviewed article.
6- الزبیدی,زهیر نجیب: هدى عبد الکریم بابان وفارس کاطع فلیح.(1996). دلیل العلاج بالأعشاب الطبیة العراقیة .شرکة آب للطباعة الغیر محدودة. بغداد. 7- شوفالیة, أندرو(2005) الطب البدیل (التداوی بالأعشاب والنباتات الطبیة)-أکادیمیة أنترناشونال – بیروت. 8-papadoyannis,Ioannis N. (1995). Determination of Datura Alkaloids by using chromatographic techniques: review Natural Toxins 3:310-316.Wiley-liss,inc.
9-Vitale, A:Acher A:Pomilio AB.(1997) Alkaloids of Datura ferox from Argentina. J. Eathano. pharmacol. Dec.1; 49(2): 81-9.
10-الربیعی, هادی مزعل (1998) تأثیر مستخلصات نبات الداتورة (Datura metel mill) فی بعض جوانب الأداء الحیاتی للذبابة المنزلیة Musca Domes, أطروحة دکتوراه فلسفة کلیة العلوم, جامعة بابل. 11-Minnsk,H.F.C. 1977 The Alkaloid Chemistry and physiology Ediled by Department of chemistry Canada volum xv.pp.14-5.
12-clark's 1986-Isolation and Identification of drugs second Edition pp.647-675.
13- محمد رمزی العمری (الکیمیاء السریریة العملی,الطبعة الثانیة,مؤسسة المعاهد الفنیة,دار الکتب للطباعة والنشر,جامعة الموصل (2001),ص93-82 . 14-YOUNG D.S.,Effect of Drugs on clinical laboratory Test 4th Ed.(1995) p. 3-498
15-TIETZ N.W.Text book of clinical chemistry,3rd ed. C.A. Burits, E.R. Ashwood, W.B. Saunders(1999) p.711-715.
16-Trived R.& Coll.riclin,chem.24,1908(1978) CAT.No.4050 Size:2×100 ml.
17-Balleter, W.G., Bushman, C.S., Tidwell,p.w., Anal. chim 33,39. | ||
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