Determination of Alliin and Allicin in different types Garlic using High Performance Liquid Chromatography
|Journal of University of Anbar for Pure Science|
|Article 6, Volume 4, Issue 2, August 2010, Pages 16-23 PDF (827.77 K)|
|Document Type: Research Paper|
|Mohammad J. Abdul Ghani*|
|University of Sallah Aldin - Collage of Basic Education|
|Abstract:Alliin and Allicin products were measured and determined by ion-pair reversed-phase liquid chromatography (RP-LC) with UV detection at 210 nm. These two Compounds were extracted from various types of garlic with methanol / ethyl acetate and chromatographed on octadecyl silane column [ODS C18 (250 x 4.6 mm id)] with gradient elution from 0.01M phosphate buffer (PH=2.5) with 5M heptanslfonic acid (mobile phaseA) to 0.01M phosphate buffer (PH=2.5) acetonitrile(1:1) (mobile phase B). Allicin was eluted after Alliin. The results observed show that the concentration differs between the different types of garlic. The aqueous Iraqi garlic extract has the highest concentration of Alliin and Allicin (17.9 ppm, 0.9%),(23.94 ppm, 1.2%) respectively. But the lowest concentration of allicin was found in French garlic extract (0.56 ppm, 0.03%) while the lowest level of Alliin was (4.3 ppm, 0.22%) in Chinese garlic extract.|
|Determination; Alliin; Allicin; Garlic; HPLC|
1.Lawson L.D., Garlic: Review of its medical effects and indicated active compounds. In Lawson L.D., and Bauer R. (eds). Phytomedicines of Eurrope: Chemistry and Biological Activity. Washington, DC: American Chemical Society 176- 209 (1998) Historical perspective on the use of garlic. Rivlin, R.S., Historical perspective on the use of garlic, J. Nutr., 131(3S), 951S-4S(2001).
2.Rabinkov et al., Biochim Biophys Acta, 1379(2):233-234, Kourounaskis, P.N., Rekka, E.A. Res Commun Chem Pathol Pharmacol, 74(2:249-52(1991).
3.Allicin. The Merk Index. 1999 by Merk&& Co Inc, Whitehouse Station, NJ, USA, Published on CD-ROM by Chapman&& Hall/CRC.
4.Cavallito C.J. and Bbailey J.H., Allicin, the antibacterial principle of Allium sativum.I. isolation physical properties and antibacterial action, J. Am. Chem.Soc., 66, 1950-1(1944).
5.Freeman F. and Kkodera Y. Garlic chemistry: stability of S-(2-propenyl)-2-propene-1-sulphinothioate(Allicin) in blood, solvents, and simulated physiological fluids,J. Agric. Food Chem., 43, 2332-8(1995).
6.Augusti K. and Mathew P.T., Lipid lowering effect of Allicin (diallyldisulfide-oxide) on long term feeding on normal rats, Experienta, 30, 468-70(1994), Han J.C. Lawson L.D., Han G., and Han P., Biochem., 157-60(1995).
7.Lawson L.D., Ransom D.K., and Huges B.G., Inhibition of whole blood platelet-aggregation by compounds in garlic clove extracts and commercial products, Thromb Res., 65, 141-56,(1992).
8.Lawson L.D., Wood S. G., and Hughes B. G., Planta Med., 57, 263-70 (1991). Release of volatile compounds from microwave heating of garlic juice with 2,4 decadienanals.J. Food chem, 64, 531-35 (1999).
9.Koch J., Berger L., Vieregge – Reiter C., Planta Med., 55, 327-31(1989).
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