Determination of Alliin and Allicin in different types Garlic using High Performance Liquid Chromatography

J. Abdul Ghani, Mohammad

doi:10.37652/juaps.2010.15391

	Determination of Alliin and Allicin in different types Garlic using High Performance Liquid Chromatography
Journal of University of Anbar for Pure Science
Article 6, Volume 4, Issue 2, August 2010, Pages 16-23 PDF (827.77 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2010.15391
Author
Mohammad J. Abdul Ghani^*
University of Sallah Aldin - Collage of Basic Education
Abstract
Abstract:Alliin and Allicin products were measured and determined by ion-pair reversed-phase liquid chromatography (RP-LC) with UV detection at 210 nm. These two Compounds were extracted from various types of garlic with methanol / ethyl acetate and chromatographed on octadecyl silane column [ODS C18 (250 x 4.6 mm id)] with gradient elution from 0.01M phosphate buffer (PH=2.5) with 5M heptanslfonic acid (mobile phaseA) to 0.01M phosphate buffer (PH=2.5) acetonitrile(1:1) (mobile phase B). Allicin was eluted after Alliin. The results observed show that the concentration differs between the different types of garlic. The aqueous Iraqi garlic extract has the highest concentration of Alliin and Allicin (17.9 ppm, 0.9%),(23.94 ppm, 1.2%) respectively. But the lowest concentration of allicin was found in French garlic extract (0.56 ppm, 0.03%) while the lowest level of Alliin was (4.3 ppm, 0.22%) in Chinese garlic extract.
Keywords
Determination; Alliin; Allicin; Garlic; HPLC

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