ISOLATION AND IDENTIFICATION OF AMYLASEPRODUCING BACTERIA FROM SOILS USING LOCAL MEDIA | ||
| Journal of University of Anbar for Pure Science | ||
| Article 12, Volume 1, Issue 2, August 2007, Pages 53-63 PDF (536.54 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2007.15369 | ||
| Author | ||
| DHAFER F. ABDUL KADER AL –RAWI* | ||
| Department of Life Sciences - College of Education - University of Anbar | ||
| Abstract | ||
| The study aimed at isolation starch hydrolyses bacteria and amylaseproducing bacteria from Ramadi soils to get efficient local isolates that produce the enzyme .30 bacteria isolates that produce the enzyme could be obtained; the (10) most efficient of which were selected. Cultural media containing plant wastes prevailing in the local environment were used. There are considered pollutants that are very cheap in the production of the enzyme. They are corn cap, clover hay Trifolium alexandrinum L., and shamblan Ceratophyllum demersum L. . The results have shown that the best production of the enzyme using starch agar was at pH7 and 35c temperature after 24 hours of incubation with the use of corn cap then shamblan as a source of carbon where the diameter of the clear zone area was 8cm from the local isolate (PR10). The local isolate (PR10) showed high ability to produce amylase enzyme using liquid cultures. The best production was at pH7 and 40c temperature using corn cap of2% concentration and incubation with the shaking incubator at 150 rpm and 1.5% inoculums concentration. The activity of the enzyme was 24.731 unit/ml using peptone as a source of nitrogen after incubation for 24 hours. Identification results of (PR10) isolate showed that it is B.subtilis bacteria. | ||
| Keywords | ||
| Bacillus subtilis | ||
| References | ||
|
[1] Ajayi A.O and Fagade OE. (2003). Utilization of cornstarch as substrate forby Bacillus subtilis. African J. of Biomedical Research, Vol. 6 (1); 37-42.
[2] Betrand Tatsinkou Fossi, Frederic Tavea, Robert Ndjouenkeu (2005). Production and Partial characterization of a thermostable amylase from ascomycetes yeast strain isolated from starchy Soils. African J. of Biotech. Vol. 4 (1), PP. 14-18.
[3] Dharani P.V.Aiyer (2004). Effect of C: N ratio on alpha amylase production by Bacillus licheniformis SPT 27. African J. of Biomedical. Vol. 3 (10), PP. 519-522.
[4] Holt, J.O., Kring, N.R., Senath, P.H..A.., Staly, J.J. and Williams, S.T. (1994). Beregey’s Manual of Determinateion Bacteriology, 9th. U.S.A.
[5] Howling, D. (1989). Mechanism of starch hydrolysis. International Bio.deterioration, 25: 15-19.
[6] Ikram-Ul-Hag, Safia Rani, Hamad Ashraf and M.A.Qadeer. (2002). Biosynthesis of Alpha Amylase by chemically treated mutant of Bacillus subtilis. Online J. of Bio. Sci. 2 (2): 73-75.
[7] Liliana N.Ceci and Jorg E.Lozano. (2002). Amylase for Apple Juice processing: Effect of pH, Heat, and Ions. Biotechnol. 40 (1) 33-38.
[8] Mamo, G., Gashe, B.A., Gessess, A. (1999). A highly thermostable amylase from a newly isolated thermophilic Bacillus sp. W11. Journal of Applied microbiology 86.
[9] Mc Carthy, A.J. (1987). Lignocellulose – degrading by actinomycetes , FEMS. Microbial. Rev. 46: 145-163.
[10] Miller, G.L. (1995). Use of the dinitrosalicylic acid reagent for the determination of reducing sugars. Anallyt. Chem. 31: 426-428.
[11] NADiRiAZ, Ikram-Ul-Hag and M.A.Qader. (2003) Characterization of by Bacillus subtilis. International Journal of Agriculture Biology. 1560-8530-3-249-252.
[12] Omemu, A.M.; Akpan, l; Bankole, M.O. and Teniola, O.D. (2005). Hydrolysis of raw tuber starches by amylase of Aspergillus niger AMO7 isolated from the soil African J. of Biotech Vol. 4 (1), PP. 19-25.
[13] Ozlem kiran, Ugur Comlekcloglu, Burhan Arikan. (2005). Effects of carbon sources and various chemicals on the production of Novel Amylase from a Thermophilic Bacillus sp. K-12, Turk J Biol. (29): 92-103.
[14] Priest, F.G. (1992). Enzymes, Extracellulare. IN. Encyclopedia of microbiology. Academic press. Inc. Vol. 2pp. 81-93.
[15] Purohit, S.S. and Mothur, S.K. (1996). Enzymes bioacceleractors In: Biotechnology, Fundamentals, and application publisher, India.
[16] Takasaki, Y. (1989). Novel maltose-Producing amylase from Bacillus megaterium G-2, Agric. Bio. Chem. 59 (2): 341-347. | ||
|
Statistics Article View: 203 PDF Download: 51 |
||