Biochemical and Molecular Identification of P.fluorescens Isolated from Iraqi Soils and Evaluation of Their Efficacy in Production of Plant Growth Promoting Compounds and Inhibitor Compounds for Macrophomina phaseolina | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 18, Issue 2, September 2018, Pages 143-157 | ||
| Authors | ||
| Abdul-Kareem E. Alkurtany; Abdullah A. Hassan; Hiba Mohammed Yousef | ||
| Abstract | ||
| 72 soil samples Were collected from the rhizosphere of (Triticum aestivum L Hordeum Sp, Vicia faba, Allium cepa, Beta vulgaris L., raphanus sativus, Solanum tuberosum, Citrullas lantus , Matricaria chmomilla, Mentha sp and Capsicum annuum) in order to isolate Pseudomonas Results showed that out of 72 soil samples, 60 isolates were belong in its charactercitic to Pseudomonas spp. Depending on the differential and biochemical tests, 48 isolates were related to P.fluorescens, Laboratory diagnosis was confirmed using a diagnostic kit APi 20E, as well as molecular identification by using the primers 16SPSEfluF and 16SPSEfluR for the amplification of the gene 16D rRNA using polymerase chain reaction PCR technique the resulting bands have molecular size of 848 base pairs of the 16S rRNA, which only amplitied the species P. fluorescens. At level of host plants highest of P. fluorescens was in the onions (83.3%) followed by barley and peas (80%) followed by wheat, potatoes, watermelon and mint (75%) then chamomile and chard and peppers (50%) and less presence was in the plant radish as the ratio reached at 42.8%. | ||
| Keywords | ||
| fluorescens Pseudomonas; Phaseolina; Growth promoting compounds; molecular identification; phaseolina | ||
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