Role of Two Different Annealing Temperatures in Genetic Variability Determination of Pseudomonas aeruginosa | ||
| Rafidain Journal of Science | ||
| Article 13, Volume 27, Issue 1, March 2018, Pages 107-113 PDF (444.46 K) | ||
| DOI: 10.33899/rjs.2018.143949 | ||
| Author | ||
| Ghada A. Mohammad | ||
| Abstract | ||
| Seven isolates of Pseudomonas aeruginosa were obtained from bacterial strain bank department of Biology/ College of Science/ Mosul University. The DNA was extracted from bacterial culture. Genetic variability was achieved by (RAPD-PCR), and ERIC2 primer was used. Two programs were used in thermal cycling containing two different annealing temperatures, the first was 50o C, its amplification and electrophoresis results showed that all seven isolates had no band except the isolate No. (3) which has revealed a band (approximately 600 bp) when compared with DNA ladder, so this temperature was not suitable for detection of genetic variation for our local bacteria. The annealing temperature for the second program was 35oC, its amplification and electrophoresis result has illustrated that there are genetic variability among just three isolates which had number 2, 3, and 4, while the other isolates have failed to show any band. So, the 35º C was better than 50º C to determine genetic variation in this study | ||
| Keywords | ||
| genetic variation; aeruginosa; RAPD; PCR; ERIC; DNA | ||
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