Presentation of Binding-Epitope of Globoseries Glycolipid Receptors is Crucial in the Binding, Colonization and Infection of Escherichia coli | ||
Almuthanna Journal of Pure Science (MJPS) | ||
Article 1, Volume 2, Issue 1, December 2014, Pages 0-0 | ||
Author | ||
Maan Abul-Milh | ||
Abstract | ||
Wild type E. coli DS 17 is a clinical isolate associated with epidemic UTI was studied. This isolate recognized selectively well Galα1-4Gal sequence of globoseries glycolipids separated on thin-layer chromatogram (TLC), coated in microtiter wells and incorporated into liposomes. E.coli DS17, representing a class II adhesin, showed high affinity to the disaccharide Galα1-4Gal sequence of the globoside glycolipid receptor in all assay systems. Weak recognition of the epitope, Galα1-4Gal, in the terminal position of globotriaosylceramide (Galα1-4Galβ4Glcβ1Cer) and also weak binding of DS17 to the disaccharide sequence within Forssman glycolipid GalNAcα3GalNAcβ3Galα1- 4Galβ4Glcβ1Cer. This strain failed to recognize glycolipids missing the disaccharide sequence like gangliotetraosylceramide Galβ3GalNAcβ4Galβ4Glcβ1Cer. The modified E. coli DS 17 by replacing class II adhesin with class III (PrsG) type, (DS17-1), bound Galα1-4Gal sequence of globoside less than Forssman glycolipid. E. coli DS17-8 strain, DS17 with the knocked out adhesin, revealed no binding to the glycolipids in all assay system. Using liposomes aggregation assay, E. coli J96, representing class I adhesin, showed strong recognition of globoside incorporated into liposomes, weakly when Forssman glycolipid was used and very weak binding to globotriaosylceramide was detected. Introduction | ||
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