Detection of IMP-Metallo Beta-lactamase in Some Gram Negative Bacteria Using Morphological and Molecular Methods | ||
| Rafidain Journal of Science | ||
| Article 22, Volume 27, Issue 1, March 2018, Pages 1-9 PDF (447.44 K) | ||
| DOI: 10.33899/rjs.2018.141067 | ||
| Authors | ||
| Sahar L. Al Saleem; Adeeba Y. Sharif | ||
| Abstract | ||
| This study detected the ability of some gram negative bacteria isolated from different clinical specimens to produce Metallo Beta Lactamase (MBL) using phenotypic and molecular methods. The ratio of the presence of these enzymes was (23.3%) using the Imipenem EDTA-Disk method and Pseudomonas aeruginosa was the highest producer 10% then Klebsiella pneumoniae (6.6%) and (3.3%) for Enterobacter colcoae, while the molecular method was used for the detection of gene for this enzyme, the results showed that Ps.aeruginosa produces the enzyme at (11.1%) while the rate of this gene produced by Acinetobacter bumannii was (5.5%) | ||
| Keywords | ||
| Metallo beta lactamase; Gram negative bacteria resistance | ||
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