Ameliorative Effect of Fenugreek on sex hormones in Polycystic Ovary Syndrome Female Rats Induced by Letrozole | ||
| Kufa Journal For Veterinary Medical Sciences | ||
| Article 1, Volume 8, Issue 2, December 2017, Pages 24-32 | ||
| Authors | ||
| Jassim M.A. Alkalby; Fouad Ziedan Hamzah | ||
| Abstract | ||
| The present study was carried out in the animal house of the College of Veterinary Medicine/ University of Basrah to investigate the role of the treatment with metformin (Met), ethanolic extract of fenugreek (FN) each alone and their combination on the sex hormones changes in polycystic ovary syndrome induced in female rats. For this purpose, the study included two experiment. First experiment aimed to determine the changes in the hormones concentration of pituitary ovarian axis associated with PCOS, induced by letrozole in rats. Fifty six adult female rats randomly divided in to two group, first group (n=16) administrated 0.5% carboxy Methyl Cellulose (CMC) 0.2ml/rat/orally by gavage and served as control group and second group (n=40) administered Letrozole (1mg/kg B.W/day) dissolved in 1ml of 0.5% carboxymethylcellulose (CMC) orally by gavage for 21 days to induced PCOS. Vaginal smear examination of all female rats was done to ensure the occurrence of PCOS. At the end of the experiment eight rats of each group were sacrificed. Blood samples were collected and serum were separated for hormonal assay. The results revealed a significant increase in serum concentration of luteinizing hormone (LH) and testosterone (T) in PCOS group compared with control group. On other hand a significant decrease in serum concentration of estrogen (E2), and progesterone (P4) in PCOS group compared with control. Second experiment: This experiment was designed to evaluate the role of metformin, fenugreek and their combination therapy in amelioration effect of sex hormones changes of induced PCOS in female rats. The remainder animals from 1st experiment (8 rats from control group and 32 rats from PCOS group) were divided into five equal subgroups including eight rats in each group as follows, First group (Negative control): Rats from the control group of first experiment were administrated of 0.2 ml/rat distilled water (D.W) / daily by oral gavage. Second group (PCOS): PCOS-induced rats administrated 0.2ml/rat D.W daily by oral gavage. Third group (PCOS+Met): PCOS-induced rats were administrated 50mg/kg B.W Met daily by oral gavage. Fourth group,(PCOS+FN): PCOS-induced rats were administrated 15mg/kg B.W FN daily by oral gavage. Fifth group (PCOS+Met+FN): PCOS-induced rats were administrated both Met and FN (50mg and 15 mg)/kg B.W respectively daily by oral gavage. The treatments were extended for 21 days.. Blood samples were collected and serum were separated for hormonal assay. The results revealed that serum concentration of LH and T still significantly higher and serum concentrations of E2 and P4 significantly lower in -Ve PCOS group compared with control after 21 days of treatment. A significant degrees of improvement were recorded in above cited parameters in all treatment groups compared with -Ve PCOS and control groups. | ||
| Keywords | ||
| fenugreek; sex hormones; PCOS; Letrozole | ||
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