STUDY THE PREVALENCE OF GIARDIASIS AND CRYPTOSPORIDIOSIS AMONG CHILDREN AT AL-RESSAFA SIDE OF BAGHDAD BY COMPARISON BETWEEN THE EFFICIENCY OF SOME DIAGNOSTIC METHODS | ||
| AL-TAQANI | ||
| Article 1, Volume 27, Issue 2, April 2014, Pages 0-0 | ||
| Authors | ||
| Noor Mamoon Khairi; Abdul Rassul Kh. Saeed | ||
| Abstract | ||
| The present study is a cross sectional study conducted on children suffered from watery diarrhea at different ages attended to some special pediatric hospitals and primary health care centers in Al-Ressafa side of Baghdad , Stool samples were collected from 166 child during the period (from the 1st.of Nov. 2012, till the end of Jan. 2013) and examined by Direct method, then they were examined using different laboratory diagnostic methods including ELISA technique ,Concentrated method by flotation technique ,and Zehil -Nelssen staining methods .ELISA technique was used to detect Giardia lamblia and Cryptosporidium spp. which is the first survey conducted in Iraq to investigate the both parasites by the same kit . The results revealed that the infection rate was (6.63%) by using direct exam compared with (10.24%) Giardia lamblia using concentrated method by flotation technique and (37.35%) with Cryptosporidium spp. by Modified Zehil-Nelssen (M.Z.N.) method, and the infection rate was (39.76%) by using ELISA technique for the both parasites. The sensitivity and specificity of ELISA technique in this study was (100%) and (64.51%) respectively when compared with direct microscopic exam, and (100%) sensitivity with ( 96.15%) specificity of ELISA compared with M.Z.N. method . The high infection rate was among children (≤5 years) old, especially at the age (≤1 year) which was (72.7%) and (36.36%) respectively . | ||
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